Team:Warsaw/Calendar-Main/2 September 2008

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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>
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<h3>Purification of proteins: A-alpha</h3><h4>Piotr, Emilia</h4>
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Piotr </h3>
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<p><ol><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0.5.</li>
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<p><ol><li>pET15b+A_alpha, pET15b+Z_alpha and pET15b+Z_omega in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0,5.</li>
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<li>Culture was induced with different concentrations of IPTG at 22&deg;C and 37&deg;C.</li>
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<li>Cultures induced with different concentrations of IPTG in 22&deg;C and 37&deg;C.</li>
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<li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p>
<li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p>

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Purification of proteins: A-alpha

Piotr, Emilia

  1. pET15b+A_alpha in Rosetta strain from overnight culture inoculated in fresh LB and cultured until OD=0.5.
  2. Culture was induced with different concentrations of IPTG at 22°C and 37°C.
  3. Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).

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