Team:Warsaw/Calendar-Main/2 September 2008

From 2008.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 3: Line 3:
<html>
<html>
-
<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4>
+
<h3>Purification of proteins: A-alpha</h3><h4>Piotr, Emilia</h4>
-
<p><ol><li>pET15b+A_alpha, pET15b+Z_alpha and pET15b+Z_omega in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0,5.</li>
+
<p><ol><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0.5.</li>
-
<li>Cultures induced with different concentrations of IPTG in 22&deg;C and 37&deg;C.</li>
+
<li>Culture was induced with different concentrations of IPTG at 22&deg;C and 37&deg;C.</li>
<li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p>
<li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p>

Latest revision as of 21:02, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha

Piotr, Emilia

  1. pET15b+A_alpha in Rosetta strain from overnight culture inoculated in fresh LB and cultured until OD=0.5.
  2. Culture was induced with different concentrations of IPTG at 22°C and 37°C.
  3. Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008"