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Team:Heidelberg/Notebook/Sensing Group/Notebook/7thweek
From 2008.igem.org
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== Tuesday, 09/16/2008 == | == Tuesday, 09/16/2008 == | ||
| - | * Digestion of Fusion 1+2 with PstI | + | * Digestion of Fusion 1+2 with PstI (NEBuffer 3 + BSA) |
[[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]] | [[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]] | ||
* F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC | * F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC | ||
Revision as of 23:41, 26 October 2008
Contents |
Tuesday, 09/16/2008
- Digestion of Fusion 1+2 with PstI (NEBuffer 3 + BSA)
- F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC
F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct
Wednesday, 09/17/2008
- Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct
- O/N cultures of LuxP cells from Glycerol stock
Thursday, 09/18/2008
- Miniprep of LuxP
- Cloning of LuxP into Fusion constructs with SalI/NotI
- Transformation into DH5a competent cells
Friday, 09/19/2008
nothing to report
