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Team:Rice University/Notebook/17 June 2008
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(Difference between revisions)
(New page: =Tuesday 17 June= *Selim Sheikh: **Prepared PCR of lambda DNA (2ul): 50 ul system ***2.5 ul of each of the designed primers STF L (forward) and STF R (reverse) ***1 ul of dNTP ***5 ul of 1...) |
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*Selim Sheikh: | *Selim Sheikh: | ||
**Prepared PCR of lambda DNA (2ul): 50 ul system | **Prepared PCR of lambda DNA (2ul): 50 ul system | ||
| - | ***2.5 ul of each of the designed primers | + | ***2.5 ul of each of the designed primers ATTP L (forward) and ATTP R (reverse) |
***1 ul of dNTP | ***1 ul of dNTP | ||
***5 ul of 10X Pfu Buffer | ***5 ul of 10X Pfu Buffer | ||
***0.5 ul of Pfu Ultra | ***0.5 ul of Pfu Ultra | ||
***36.5 ul of dH2O | ***36.5 ul of dH2O | ||
Latest revision as of 21:02, 18 June 2008
Tuesday 17 June
- Selim Sheikh:
- Prepared PCR of lambda DNA (2ul): 50 ul system
- 2.5 ul of each of the designed primers ATTP L (forward) and ATTP R (reverse)
- 1 ul of dNTP
- 5 ul of 10X Pfu Buffer
- 0.5 ul of Pfu Ultra
- 36.5 ul of dH2O
- Prepared PCR of lambda DNA (2ul): 50 ul system
