Team:Warsaw/Calendar-Main/10 October 2008
From 2008.igem.org
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<h3>Visit in US Embassy</h3> | <h3>Visit in US Embassy</h3> | ||
<p>Visas have been accorded to the whole team.</p> | <p>Visas have been accorded to the whole team.</p> | ||
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+ | <h3>Preparation of pT7_alpha_link</h3> | ||
+ | <h4>Michał K.</h4> | ||
+ | <ol> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest reaction. </li> | ||
+ | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of | ||
+ | <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>ΔA (BBa_K103003)</a> with EcoRI and SacI (BamHI buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> with CIAP </li> | ||
+ | <li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 2200 bp. </li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>ΔA (BBa_K103003)</a> with pT7_alpha fragment (1 hr).</li> | ||
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Revision as of 09:00, 27 October 2008
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Visit in US EmbassyVisas have been accorded to the whole team. Preparation of pT7_alpha_linkMichał K.
Preparation of BioBricksMichał K.PiotrMichał K.
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