Team:Warsaw/Calendar-Main/29 September 2008
From 2008.igem.org
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<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103006>OmpA_linker (BBa_K103006)</a> (from 18 September). </li></ol> | <li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103006>OmpA_linker (BBa_K103006)</a> (from 18 September). </li></ol> | ||
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+ | <h3>Preparation of pT7_omega_link</h3> | ||
+ | <h4>Michał K.</h4> | ||
+ | <ol> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega">pACYC177 + OmpA_omega</a> plasmid using | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegLNde">OmegLNde</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinP_BS">LinP_BS</a> primers (annealing temperature 55 °C; elongation length 60s) to obtain omega_link fragment. </li> | ||
+ | <li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (omega_linker - 350 bp) (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008#fig1">Fig. 1.</a>).</li></ol> | ||
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<li> Gel electrophoresis of PCR products <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008#fig1">Fig. 1</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (ΔA - 250 bp, pLac_OmpA_omega_ - 1200 bp, omega_linker - 350 bp and OmpA_omega_ - 900 bp).</li> | <li> Gel electrophoresis of PCR products <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008#fig1">Fig. 1</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (ΔA - 250 bp, pLac_OmpA_omega_ - 1200 bp, omega_linker - 350 bp and OmpA_omega_ - 900 bp).</li> | ||
Revision as of 09:40, 27 October 2008
MutD5 testingPiotrSequencing results: there weren't any mutations in plasmids isolated from MutD5 - maybe this strain is too weak as a mutator. Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of pSB1A3+OmpA-linker (BBa_K103006)Michał K.
Preparation of pT7_omega_linkMichał K.
Preparation of BioBricksMichał K.
Preparation of vectors for BiobricksPiotrInoculation of bacteria received from iGEM HQs, carrying pSB2K3 and BBa_I739204 (pACYC177 converted into BioBrick vector) plasmids. Fig. 1. PCR on pLac_OmpA_omega_1. Marker 2. PCR product
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