Team:Warsaw/Calendar-Main/20 October 2008

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<h3>Preparation of pT7_alpha_link</h3>
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<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<p>Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + pT7_alpha to LB with kanamycin.</p>
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<p>Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a> to LB with kanamycin.</p>

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Preparation of pSB1A3 + alpha_linker under PT7 (BBa_K103019)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of BioBricks

Michał K.

  1. Inoculation of colonies from plate with ligation of 5kb(?????) + pT7_alpha to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.
  2. Digest of 3kb(????)+pLac_OmpA_alpha and RFP(????)+OmpA with EcoRI and SacI (BamHI buffer).
  3. Gel electrophoresis and gel-out of proper band 500 bp for RFP(????)+OmpA and 3000 bp for 3kb(????)+pLac_OmpA_alpha.
  4. Ligation of DNA fragments: 3kb(?????)_ alpha + Omp.

Piotr

  1. Transformation of TOP10 with above ligation.
  2. Tranformants planting on LB with kanamycin.

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