Team:Warsaw/Calendar-Main/22 October 2008

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<h3>Preparation of AraC+pBAD+AID</h3>
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<h4>Michał K.</h4>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + AraC+pBAD+AID.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.</li></ol>
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<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>

Revision as of 11:44, 27 October 2008

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Preparation of AraC+pBAD+AID

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AraC+pBAD+AID.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of BioBricks

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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