Team:UNIPV-Pavia/Parts

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-
{|border="1" width="100%"
+
{|border="1" width="60%"
!New Part
!New Part
!Notebook Name
!Notebook Name
Line 31: Line 31:
|''-''
|''-''
|01
|01
-
|BBa_J23100-BBa_E0040
+
|J23100-E0240
|Pcon-GFP_protein_generator
|Pcon-GFP_protein_generator
-
|BBa_J61002
+
|J61002
|Test
|Test
|-
|-
-
|''-''
+
|<font color="green">'''K081005'''</font>
-
|02
+
|<font color="green">'''02'''</font>
-
|BBa_J23100-BBa_B0030
+
|<font color="green">'''J23100-B0030'''</font>
-
|Pcon-RBS
+
|<font color="green">'''Pcon-RBS'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081006'''</font>
-
|03
+
|<font color="green">'''03'''</font>
-
|BBa_R0051-BBa_B0030
+
|<font color="green">'''R0051-B0030'''</font>
-
|Plambda-RBS
+
|<font color="green">'''Plambda-RBS'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''S04119'''</font>
-
|04
+
|<font color="green">'''04'''</font>
-
|BBa_B0030-BBa_E0040
+
|<font color="green">'''B0030-E0040'''</font>
-
|RBS-GFP
+
|<font color="green">'''RBS-GFP'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081007'''</font>
-
|05
+
|<font color="green">'''05'''</font>
-
|BBa_B0030-BBa_C0051
+
|<font color="green">'''B0030-C0051'''</font>
-
|RBS-cI
+
|<font color="green">'''RBS-cI'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''S04120'''</font>
-
|06
+
|<font color="green">'''06'''</font>
-
|BBa_J23100-BBa_E1010
+
|<font color="green">'''J23100-E1010'''</font>
-
|RBS-RFP
+
|<font color="green">'''RBS-RFP'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081008'''</font>
-
|07
+
|<font color="green">'''07'''</font>
-
|BBa_B0030-BBa_C0061
+
|<font color="green">'''B0030-C0061'''</font>
-
|RBS-luxI
+
|<font color="green">'''RBS-luxI'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081009'''</font>
-
|08
+
|<font color="green">'''08'''</font>
-
|BBa_B0030-BBa_C0078
+
|<font color="green">'''B0030-C0078'''</font>
-
|RBS-lasI
+
|<font color="green">'''RBS-lasI'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|style="background:gray"|''-''
-
|09
+
|style="background:gray"|09
-
|BBa_J23100-BBa_B0030-BBa_C0012
+
|style="background:gray"|K081005-C0012
-
|Pcon-RBS-lacI
+
|style="background:gray"|Pcon-RBS-lacI
-
|pSB1A2
+
|style="background:gray"|pSB1A2
-
|Intermediate
+
|style="background:gray"|Intermediate
|-
|-
-
|''-''
+
|''K081010''
|10
|10
-
|BBa_J23100-BBa_B0030-BBa_C0040
+
|K081005-C0040
|Pcon-RBS-tetR
|Pcon-RBS-tetR
|pSB1A2
|pSB1A2
|Intermediate
|Intermediate
|-
|-
-
|''-''
+
|style="background:red; color:white"|''-''
-
|11
+
|style="background:red; color:white"|11
-
|BBa_J23100-BBa_B0030-BBa_I15010
+
|style="background:red; color:white"|K081005-I15010
-
|Pcon-RBS-cph8
+
|style="background:red; color:white"|Pcon-RBS-cph8
-
|pSB1A2
+
|style="background:red; color:white"|pSB1A2
-
|Intermediate
+
|style="background:red; color:white"|Intermediate
|-
|-
-
|''-''
+
|<font color="green">'''K081011'''</font>
-
|12
+
|<font color="green">'''12'''</font>
-
|BBa_R0051-BBa_B0030-BBa_C0062
+
|<font color="green">'''K081006-C0062'''</font>
-
|Plambda-RBS-luxR
+
|<font color="green">'''Plambda-RBS-luxR'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081012'''</font>
-
|13
+
|<font color="green">'''13'''</font>
-
|BBa_B0030-BBa_E0040-BBa_B1006
+
|<font color="green">'''S04119-B1006'''</font>
-
|RBS-GFP-T
+
|<font color="green">'''RBS-GFP-T'''</font>
-
|pSB1AK3
+
|<font color="green">'''pSB1AK3'''</font>
-
|GFP protein generator with 39bp artificial terminator
+
|<font color="green">'''GFP protein generator with 39bp artificial terminator'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081013'''</font>
-
|14
+
|<font color="green">'''14'''</font>
-
|BBa_B0030-BBa_C0051-BBa_B0030
+
|<font color="green">'''K081007-B0030'''</font>
-
|RBS-cI-RBS
+
|<font color="green">'''RBS-cI-RBS'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|Intermediate
+
|<font color="green">'''Intermediate'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081014'''</font>
-
|15
+
|<font color="green">'''15'''</font>
-
|BBa_B0030-BBa_E1010-BBa_B1006
+
|<font color="green">'''S04120-B1006'''</font>
-
|RBS-RFP-T
+
|<font color="green">'''RBS-RFP-T'''</font>
-
|pSB1AK3
+
|<font color="green">'''pSB1AK3'''</font>
-
|GFP protein generator with 39bp artificial terminator
+
|<font color="green">'''GFP protein generator with 39bp artificial terminator'''</font>
|-
|-
-
|''-''
+
|''K081015''
|16
|16
-
|BBa_B0030-BBa_C0061-BBa_B1006
+
|K081008-B1006
|RBS-luxI-T
|RBS-luxI-T
|pSB1AK3
|pSB1AK3
|luxI protein generator RCT
|luxI protein generator RCT
|-
|-
-
|''-''
+
|''K081016''
|17
|17
-
|BBa_B0030-BBa_C0078-BBa_B1006
+
|K081009-B1006
|RBS-lasI-T
|RBS-lasI-T
|pSB1AK3
|pSB1AK3
|lasI protein generator RCT
|lasI protein generator RCT
|-
|-
-
|''-''
+
|''K081017''
|18
|18
-
|BBa_B0030-BBa_I15009
+
|B0030-I15009
|RBS-pcyA
|RBS-pcyA
|pSB1A2
|pSB1A2
|Intermediate
|Intermediate
|-
|-
-
|''-''
+
|style="background:gray"|''-''
-
|19
+
|style="background:gray"|19
-
|BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006
+
|style="background:gray"|(K081005-C0012)-B1006
-
|Pcon-RBS-lacI-T
+
|style="background:gray"|Pcon-RBS-lacI-T
-
|pSB1AK3
+
|style="background:gray"|pSB1AK3
-
|lacI protein generator PRCT
+
|style="background:gray"|lacI protein generator PRCT
|-
|-
-
|''-''
+
|<font color="green">'''K081018'''</font>
-
|20
+
|<font color="green">'''20'''</font>
-
|BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006
+
|<font color="green">'''K081010-B1006'''</font>
-
|Pcon-RBS-tetR-T
+
|<font color="green">'''Pcon-RBS-tetR-T'''</font>
-
|pSB1AK3
+
|<font color="green">'''pSB1AK3'''</font>
-
|tetR protein generator PCRT
+
|<font color="green">'''tetR protein generator PCRT'''</font>
|-
|-
-
|''-''
+
|style="background:red; color:white"|''-''
-
|21
+
|style="background:red; color:white"|21
-
|BBa_J23100-BBa_B0030-BBa_I15010-BBa_B1006
+
|style="background:red; color:white"|(K081005-I15010)-B0030
-
|Pcon-RBS-cph8-T
+
|style="background:red; color:white"|Pcon-RBS-cph8-RBS
-
|pSB1AK3
+
|style="background:red; color:white"|?
-
|cph8 protein generator PRCT
+
|style="background:red; color:white"|Intermediate
|-
|-
-
|''-''
+
|<font color="green">'''K081019'''</font>
-
|22
+
|<font color="green">'''22'''</font>
-
|BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
+
|<font color="green">'''K081011-B1006'''</font>
-
|Plambda-RBS-luxR-T
+
|<font color="green">'''Plambda-RBS-luxR-T'''</font>
-
|pSB1AK3
+
|<font color="green">'''pSB1AK3'''</font>
-
|Plambda regulated luxR protein generator PRCT
+
|<font color="green">'''Plambda regulated luxR protein generator PRCT'''</font>
|-
|-
-
|''-''
+
|''K081020''
|23
|23
-
|BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079
+
|K081013-C0079
|RBS-cI-RBS-lasR
|RBS-cI-RBS-lasR
|pSB1A2
|pSB1A2
|Intermediate
|Intermediate
|-
|-
-
|24
+
|<font color="green">'''K081000'''</font>
-
|BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062
+
|<font color="green">'''24'''</font>
-
|RBS-luxI-T-Plux
+
|<font color="green">'''K081015-R0062'''</font>
-
|pSB1A2
+
|<font color="green">'''RBS-luxI-T-Plux'''</font>
-
|'''Mux - Channel 0'''
+
|<font color="green">'''pSB1A2'''</font>
 +
|<font color="green">'''Mux - Channel 0'''</font>
|-
|-
-
|''-''
+
|<font color="green">'''K081001'''</font>
-
|25
+
|<font color="green">'''25'''</font>
-
|BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079
+
|<font color="green">'''K081016-R0079'''</font>
-
|RBS-lasI-T-Plas
+
|<font color="green">'''RBS-lasI-T-Plas'''</font>
-
|pSB1A2
+
|<font color="green">'''pSB1A2'''</font>
-
|'''Mux - Channel 1'''
+
|<font color="green">'''Mux - Channel 1'''</font>
|-
|-
-
|''-''
+
|''K081021''
|26
|26
-
|BBa_B0030-BBa_I15009-BBa_B1006
+
|K081017-B1006
|RBS-pcyA-T
|RBS-pcyA-T
|pSB1AK3
|pSB1AK3
|pcyA protein generator RCT
|pcyA protein generator RCT
|-
|-
-
|''-''
+
|style="background:gray"|''-''
-
|27
+
|style="background:gray"|27
-
|BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006-BBa_R0010
+
|style="background:gray"|(K081005-C0012-B1006)-R0010
-
|Pcon-RBS-lacI-T-Plac
+
|style="background:gray"|Pcon-RBS-lacI-T-Plac
-
|pSB1AK3
+
|style="background:gray"|pSB1AK3
-
|lacI composite part: PRCT-Plac
+
|style="background:gray"|lacI composite part: PRCT-Plac
 +
|-
 +
|style="background:gray"|''-''
 +
|style="background:gray"|28
 +
|style="background:gray"|K081018-R0040
 +
|style="background:gray"|Pcon-RBS-tetR-T-Ptet
 +
|style="background:gray"|pSB1A2
 +
|style="background:gray"|tetR composite part: PRCT-Ptet
|-
|-
-
|''-''
+
|style="background:red; color:white"|''-''
-
|28
+
|style="background:red; color:white"|29
-
|BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040
+
|style="background:red; color:white"|(K081005-I15010-B0030)-I15008
-
|Pcon-RBS-tetR-T-Ptet
+
|style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1
 +
|style="background:red; color:white"|?
 +
|style="background:red; color:white"|Intermediate
 +
|-
 +
|<font color="red">'''K081022'''</font>
 +
|<font color="red">'''30'''</font>
 +
|<font color="red">'''K081019-R0062'''</font>
 +
|<font color="red">'''Plambda-RBS-luxR-T-Plux'''</font>
 +
|<font color="red">'''pSB1A2'''</font>
 +
|<font color="red">'''luxR composite part: Plambda regulated PRCT-Plux'''</font>
 +
|-
 +
|<font color="blue">'''K081023'''</font>
 +
|<font color="blue">'''31'''</font>
 +
|<font color="blue">'''K081020-B1006'''</font>
 +
|<font color="blue">'''RBS-cI-RBS-lasR-T'''</font>
 +
|<font color="blue">'''pSB1AK3'''</font>
 +
|<font color="blue">'''cI-lasR protein generator RCRCT'''</font>
 +
|-
 +
|''K081024''
 +
|32
 +
|K081021-R0082
 +
|RBS-pcyA-T-Pomp
|pSB1A2
|pSB1A2
-
|tetR composite part: PRCT-Ptet
+
|pcyA composite part: RCT-Pomp
 +
|-
 +
|style="background:red; color:white"|''-''
 +
|style="background:red; color:white"|33
 +
|style="background:red; color:white"|(K081005-I15010-B0030-I15008)-K081024
 +
|style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp
 +
|style="background:red; color:white"|?
 +
|style="background:red; color:white"|Light responsive system under constitutive promoter: PRCRCRCT-Pomp
 +
|-
 +
|<font color="red">'''K081004'''</font>
 +
|<font color="red">'''34'''</font>
 +
|<font color="red">'''K081023-K081022'''</font>
 +
|<font color="red">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux'''</font>
 +
|<font color="red">'''pSB1AK3'''</font>
 +
|<font color="red">'''Demux - Selector'''</font>
 +
|-
 +
|<font color="blue">'''K081002'''</font>
 +
|<font color="blue">'''35'''</font>
 +
|<font color="blue">'''K081023-K081019'''</font>
 +
|<font color="blue">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T'''</font>
 +
|<font color="blue">'''pSB1AK3'''</font>
 +
|<font color="blue">'''Mux - Selector'''</font>
|-
|-
 +
|<font color="blue">'''K081003'''</font>
 +
|<font color="blue">'''36'''</font>
 +
|<font color="blue">'''K081008-K081001'''</font>
 +
|<font color="blue">'''RBS-luxI-RBS-lasI-T-Plas'''</font>
 +
|<font color="blue">'''pSB1A2'''</font>
 +
|<font color="blue">'''Demux - Input'''</font>
 +
|}
 +
 +
<br>
 +
*01 part has not been submitted to the registry because it was a test assembly.
 +
*<font color="red">'''Red'''</font>-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
 +
*<font color="green">'''Green'''</font>-coloured parts have been succesfully sequenced.
 +
*<font color="blue">'''Blue'''</font>-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct.
 +
*<font color="red">'''Red'''</font>-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts seemed to work and so we decided to submit them.
 +
*24, 25, 34, 35, 36 are parts of our final Mux or Demux systems.
 +
*Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages!
 +
*Parts in <font color="gray">'''gray'''</font> were programmed, but they have not been assembled and so they haven't been submitted.

Latest revision as of 14:36, 27 October 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook


Assembled Parts Notation

In notebook section we sometimes use abbreviations to name assembled parts. New parts' official name, notebook abbreviations and new parts description are reported in the following table:


New Part Notebook Name Assembled Parts(code) Assembled Parts(name) Vector Description
- 01 J23100-E0240 Pcon-GFP_protein_generator J61002 Test
K081005 02 J23100-B0030 Pcon-RBS pSB1A2 Intermediate
K081006 03 R0051-B0030 Plambda-RBS pSB1A2 Intermediate
S04119 04 B0030-E0040 RBS-GFP pSB1A2 Intermediate
K081007 05 B0030-C0051 RBS-cI pSB1A2 Intermediate
S04120 06 J23100-E1010 RBS-RFP pSB1A2 Intermediate
K081008 07 B0030-C0061 RBS-luxI pSB1A2 Intermediate
K081009 08 B0030-C0078 RBS-lasI pSB1A2 Intermediate
- 09 K081005-C0012 Pcon-RBS-lacI pSB1A2 Intermediate
K081010 10 K081005-C0040 Pcon-RBS-tetR pSB1A2 Intermediate
- 11 K081005-I15010 Pcon-RBS-cph8 pSB1A2 Intermediate
K081011 12 K081006-C0062 Plambda-RBS-luxR pSB1A2 Intermediate
K081012 13 S04119-B1006 RBS-GFP-T pSB1AK3 GFP protein generator with 39bp artificial terminator
K081013 14 K081007-B0030 RBS-cI-RBS pSB1A2 Intermediate
K081014 15 S04120-B1006 RBS-RFP-T pSB1AK3 GFP protein generator with 39bp artificial terminator
K081015 16 K081008-B1006 RBS-luxI-T pSB1AK3 luxI protein generator RCT
K081016 17 K081009-B1006 RBS-lasI-T pSB1AK3 lasI protein generator RCT
K081017 18 B0030-I15009 RBS-pcyA pSB1A2 Intermediate
- 19 (K081005-C0012)-B1006 Pcon-RBS-lacI-T pSB1AK3 lacI protein generator PRCT
K081018 20 K081010-B1006 Pcon-RBS-tetR-T pSB1AK3 tetR protein generator PCRT
- 21 (K081005-I15010)-B0030 Pcon-RBS-cph8-RBS ? Intermediate
K081019 22 K081011-B1006 Plambda-RBS-luxR-T pSB1AK3 Plambda regulated luxR protein generator PRCT
K081020 23 K081013-C0079 RBS-cI-RBS-lasR pSB1A2 Intermediate
K081000 24 K081015-R0062 RBS-luxI-T-Plux pSB1A2 Mux - Channel 0
K081001 25 K081016-R0079 RBS-lasI-T-Plas pSB1A2 Mux - Channel 1
K081021 26 K081017-B1006 RBS-pcyA-T pSB1AK3 pcyA protein generator RCT
- 27 (K081005-C0012-B1006)-R0010 Pcon-RBS-lacI-T-Plac pSB1AK3 lacI composite part: PRCT-Plac
- 28 K081018-R0040 Pcon-RBS-tetR-T-Ptet pSB1A2 tetR composite part: PRCT-Ptet
- 29 (K081005-I15010-B0030)-I15008 Pcon-RBS-cph8-RBS-ho1 ? Intermediate
K081022 30 K081019-R0062 Plambda-RBS-luxR-T-Plux pSB1A2 luxR composite part: Plambda regulated PRCT-Plux
K081023 31 K081020-B1006 RBS-cI-RBS-lasR-T pSB1AK3 cI-lasR protein generator RCRCT
K081024 32 K081021-R0082 RBS-pcyA-T-Pomp pSB1A2 pcyA composite part: RCT-Pomp
- 33 (K081005-I15010-B0030-I15008)-K081024 Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp ? Light responsive system under constitutive promoter: PRCRCRCT-Pomp
K081004 34 K081023-K081022 RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux pSB1AK3 Demux - Selector
K081002 35 K081023-K081019 RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T pSB1AK3 Mux - Selector
K081003 36 K081008-K081001 RBS-luxI-RBS-lasI-T-Plas pSB1A2 Demux - Input


  • 01 part has not been submitted to the registry because it was a test assembly.
  • Red-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
  • Green-coloured parts have been succesfully sequenced.
  • Blue-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct.
  • Red-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts seemed to work and so we decided to submit them.
  • 24, 25, 34, 35, 36 are parts of our final Mux or Demux systems.
  • Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages!
  • Parts in gray were programmed, but they have not been assembled and so they haven't been submitted.
Retrieved from "http://2008.igem.org/Team:UNIPV-Pavia/Parts"