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Team:UNIPV-Pavia/Parts
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| - | {|border="1" width=" | + | {|border="1" width="60%" |
!New Part | !New Part | ||
!Notebook Name | !Notebook Name | ||
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|<font color="green">'''Intermediate'''</font> | |<font color="green">'''Intermediate'''</font> | ||
|- | |- | ||
| - | |<font color="green">''' | + | |<font color="green">'''S04120'''</font> |
|<font color="green">'''06'''</font> | |<font color="green">'''06'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''J23100-E1010'''</font> |
|<font color="green">'''RBS-RFP'''</font> | |<font color="green">'''RBS-RFP'''</font> | ||
|<font color="green">'''pSB1A2'''</font> | |<font color="green">'''pSB1A2'''</font> | ||
| Line 87: | Line 87: | ||
|style="background:gray"|''-'' | |style="background:gray"|''-'' | ||
|style="background:gray"|09 | |style="background:gray"|09 | ||
| - | |style="background:gray"| | + | |style="background:gray"|K081005-C0012 |
|style="background:gray"|Pcon-RBS-lacI | |style="background:gray"|Pcon-RBS-lacI | ||
|style="background:gray"|pSB1A2 | |style="background:gray"|pSB1A2 | ||
| Line 94: | Line 94: | ||
|''K081010'' | |''K081010'' | ||
|10 | |10 | ||
| - | | | + | |K081005-C0040 |
|Pcon-RBS-tetR | |Pcon-RBS-tetR | ||
|pSB1A2 | |pSB1A2 | ||
| Line 101: | Line 101: | ||
|style="background:red; color:white"|''-'' | |style="background:red; color:white"|''-'' | ||
|style="background:red; color:white"|11 | |style="background:red; color:white"|11 | ||
| - | |style="background:red; color:white"| | + | |style="background:red; color:white"|K081005-I15010 |
|style="background:red; color:white"|Pcon-RBS-cph8 | |style="background:red; color:white"|Pcon-RBS-cph8 | ||
|style="background:red; color:white"|pSB1A2 | |style="background:red; color:white"|pSB1A2 | ||
| Line 108: | Line 108: | ||
|<font color="green">'''K081011'''</font> | |<font color="green">'''K081011'''</font> | ||
|<font color="green">'''12'''</font> | |<font color="green">'''12'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081006-C0062'''</font> |
|<font color="green">'''Plambda-RBS-luxR'''</font> | |<font color="green">'''Plambda-RBS-luxR'''</font> | ||
|<font color="green">'''pSB1A2'''</font> | |<font color="green">'''pSB1A2'''</font> | ||
| Line 115: | Line 115: | ||
|<font color="green">'''K081012'''</font> | |<font color="green">'''K081012'''</font> | ||
|<font color="green">'''13'''</font> | |<font color="green">'''13'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''S04119-B1006'''</font> |
|<font color="green">'''RBS-GFP-T'''</font> | |<font color="green">'''RBS-GFP-T'''</font> | ||
|<font color="green">'''pSB1AK3'''</font> | |<font color="green">'''pSB1AK3'''</font> | ||
| Line 122: | Line 122: | ||
|<font color="green">'''K081013'''</font> | |<font color="green">'''K081013'''</font> | ||
|<font color="green">'''14'''</font> | |<font color="green">'''14'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081007-B0030'''</font> |
|<font color="green">'''RBS-cI-RBS'''</font> | |<font color="green">'''RBS-cI-RBS'''</font> | ||
|<font color="green">'''pSB1A2'''</font> | |<font color="green">'''pSB1A2'''</font> | ||
| Line 129: | Line 129: | ||
|<font color="green">'''K081014'''</font> | |<font color="green">'''K081014'''</font> | ||
|<font color="green">'''15'''</font> | |<font color="green">'''15'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''S04120-B1006'''</font> |
|<font color="green">'''RBS-RFP-T'''</font> | |<font color="green">'''RBS-RFP-T'''</font> | ||
|<font color="green">'''pSB1AK3'''</font> | |<font color="green">'''pSB1AK3'''</font> | ||
| Line 136: | Line 136: | ||
|''K081015'' | |''K081015'' | ||
|16 | |16 | ||
| - | | | + | |K081008-B1006 |
|RBS-luxI-T | |RBS-luxI-T | ||
|pSB1AK3 | |pSB1AK3 | ||
| Line 143: | Line 143: | ||
|''K081016'' | |''K081016'' | ||
|17 | |17 | ||
| - | | | + | |K081009-B1006 |
|RBS-lasI-T | |RBS-lasI-T | ||
|pSB1AK3 | |pSB1AK3 | ||
| Line 157: | Line 157: | ||
|style="background:gray"|''-'' | |style="background:gray"|''-'' | ||
|style="background:gray"|19 | |style="background:gray"|19 | ||
| - | |style="background:gray"| | + | |style="background:gray"|(K081005-C0012)-B1006 |
|style="background:gray"|Pcon-RBS-lacI-T | |style="background:gray"|Pcon-RBS-lacI-T | ||
|style="background:gray"|pSB1AK3 | |style="background:gray"|pSB1AK3 | ||
| Line 164: | Line 164: | ||
|<font color="green">'''K081018'''</font> | |<font color="green">'''K081018'''</font> | ||
|<font color="green">'''20'''</font> | |<font color="green">'''20'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081010-B1006'''</font> |
|<font color="green">'''Pcon-RBS-tetR-T'''</font> | |<font color="green">'''Pcon-RBS-tetR-T'''</font> | ||
|<font color="green">'''pSB1AK3'''</font> | |<font color="green">'''pSB1AK3'''</font> | ||
| Line 171: | Line 171: | ||
|style="background:red; color:white"|''-'' | |style="background:red; color:white"|''-'' | ||
|style="background:red; color:white"|21 | |style="background:red; color:white"|21 | ||
| - | |style="background:red; color:white"| | + | |style="background:red; color:white"|(K081005-I15010)-B0030 |
|style="background:red; color:white"|Pcon-RBS-cph8-RBS | |style="background:red; color:white"|Pcon-RBS-cph8-RBS | ||
|style="background:red; color:white"|? | |style="background:red; color:white"|? | ||
| Line 178: | Line 178: | ||
|<font color="green">'''K081019'''</font> | |<font color="green">'''K081019'''</font> | ||
|<font color="green">'''22'''</font> | |<font color="green">'''22'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081011-B1006'''</font> |
|<font color="green">'''Plambda-RBS-luxR-T'''</font> | |<font color="green">'''Plambda-RBS-luxR-T'''</font> | ||
|<font color="green">'''pSB1AK3'''</font> | |<font color="green">'''pSB1AK3'''</font> | ||
| Line 185: | Line 185: | ||
|''K081020'' | |''K081020'' | ||
|23 | |23 | ||
| - | | | + | |K081013-C0079 |
|RBS-cI-RBS-lasR | |RBS-cI-RBS-lasR | ||
|pSB1A2 | |pSB1A2 | ||
| Line 192: | Line 192: | ||
|<font color="green">'''K081000'''</font> | |<font color="green">'''K081000'''</font> | ||
|<font color="green">'''24'''</font> | |<font color="green">'''24'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081015-R0062'''</font> |
|<font color="green">'''RBS-luxI-T-Plux'''</font> | |<font color="green">'''RBS-luxI-T-Plux'''</font> | ||
|<font color="green">'''pSB1A2'''</font> | |<font color="green">'''pSB1A2'''</font> | ||
| Line 199: | Line 199: | ||
|<font color="green">'''K081001'''</font> | |<font color="green">'''K081001'''</font> | ||
|<font color="green">'''25'''</font> | |<font color="green">'''25'''</font> | ||
| - | |<font color="green">''' | + | |<font color="green">'''K081016-R0079'''</font> |
|<font color="green">'''RBS-lasI-T-Plas'''</font> | |<font color="green">'''RBS-lasI-T-Plas'''</font> | ||
|<font color="green">'''pSB1A2'''</font> | |<font color="green">'''pSB1A2'''</font> | ||
| Line 206: | Line 206: | ||
|''K081021'' | |''K081021'' | ||
|26 | |26 | ||
| - | | | + | |K081017-B1006 |
|RBS-pcyA-T | |RBS-pcyA-T | ||
|pSB1AK3 | |pSB1AK3 | ||
| Line 213: | Line 213: | ||
|style="background:gray"|''-'' | |style="background:gray"|''-'' | ||
|style="background:gray"|27 | |style="background:gray"|27 | ||
| - | |style="background:gray"| | + | |style="background:gray"|(K081005-C0012-B1006)-R0010 |
|style="background:gray"|Pcon-RBS-lacI-T-Plac | |style="background:gray"|Pcon-RBS-lacI-T-Plac | ||
|style="background:gray"|pSB1AK3 | |style="background:gray"|pSB1AK3 | ||
| Line 220: | Line 220: | ||
|style="background:gray"|''-'' | |style="background:gray"|''-'' | ||
|style="background:gray"|28 | |style="background:gray"|28 | ||
| - | |style="background:gray"| | + | |style="background:gray"|K081018-R0040 |
|style="background:gray"|Pcon-RBS-tetR-T-Ptet | |style="background:gray"|Pcon-RBS-tetR-T-Ptet | ||
|style="background:gray"|pSB1A2 | |style="background:gray"|pSB1A2 | ||
| Line 227: | Line 227: | ||
|style="background:red; color:white"|''-'' | |style="background:red; color:white"|''-'' | ||
|style="background:red; color:white"|29 | |style="background:red; color:white"|29 | ||
| - | |style="background:red; color:white"| | + | |style="background:red; color:white"|(K081005-I15010-B0030)-I15008 |
|style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1 | |style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1 | ||
|style="background:red; color:white"|? | |style="background:red; color:white"|? | ||
| Line 234: | Line 234: | ||
|<font color="red">'''K081022'''</font> | |<font color="red">'''K081022'''</font> | ||
|<font color="red">'''30'''</font> | |<font color="red">'''30'''</font> | ||
| - | |<font color="red">''' | + | |<font color="red">'''K081019-R0062'''</font> |
|<font color="red">'''Plambda-RBS-luxR-T-Plux'''</font> | |<font color="red">'''Plambda-RBS-luxR-T-Plux'''</font> | ||
|<font color="red">'''pSB1A2'''</font> | |<font color="red">'''pSB1A2'''</font> | ||
| Line 241: | Line 241: | ||
|<font color="blue">'''K081023'''</font> | |<font color="blue">'''K081023'''</font> | ||
|<font color="blue">'''31'''</font> | |<font color="blue">'''31'''</font> | ||
| - | |<font color="blue">''' | + | |<font color="blue">'''K081020-B1006'''</font> |
|<font color="blue">'''RBS-cI-RBS-lasR-T'''</font> | |<font color="blue">'''RBS-cI-RBS-lasR-T'''</font> | ||
|<font color="blue">'''pSB1AK3'''</font> | |<font color="blue">'''pSB1AK3'''</font> | ||
| Line 248: | Line 248: | ||
|''K081024'' | |''K081024'' | ||
|32 | |32 | ||
| - | | | + | |K081021-R0082 |
|RBS-pcyA-T-Pomp | |RBS-pcyA-T-Pomp | ||
|pSB1A2 | |pSB1A2 | ||
| Line 255: | Line 255: | ||
|style="background:red; color:white"|''-'' | |style="background:red; color:white"|''-'' | ||
|style="background:red; color:white"|33 | |style="background:red; color:white"|33 | ||
| - | |style="background:red; color:white"| | + | |style="background:red; color:white"|(K081005-I15010-B0030-I15008)-K081024 |
|style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp | |style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp | ||
|style="background:red; color:white"|? | |style="background:red; color:white"|? | ||
| Line 262: | Line 262: | ||
|<font color="red">'''K081004'''</font> | |<font color="red">'''K081004'''</font> | ||
|<font color="red">'''34'''</font> | |<font color="red">'''34'''</font> | ||
| - | |<font color="red">''' | + | |<font color="red">'''K081023-K081022'''</font> |
|<font color="red">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux'''</font> | |<font color="red">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux'''</font> | ||
|<font color="red">'''pSB1AK3'''</font> | |<font color="red">'''pSB1AK3'''</font> | ||
| Line 269: | Line 269: | ||
|<font color="blue">'''K081002'''</font> | |<font color="blue">'''K081002'''</font> | ||
|<font color="blue">'''35'''</font> | |<font color="blue">'''35'''</font> | ||
| - | |<font color="blue">''' | + | |<font color="blue">'''K081023-K081019'''</font> |
|<font color="blue">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T'''</font> | |<font color="blue">'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T'''</font> | ||
|<font color="blue">'''pSB1AK3'''</font> | |<font color="blue">'''pSB1AK3'''</font> | ||
| Line 276: | Line 276: | ||
|<font color="blue">'''K081003'''</font> | |<font color="blue">'''K081003'''</font> | ||
|<font color="blue">'''36'''</font> | |<font color="blue">'''36'''</font> | ||
| - | |<font color="blue">''' | + | |<font color="blue">'''K081008-K081001'''</font> |
|<font color="blue">'''RBS-luxI-RBS-lasI-T-Plas'''</font> | |<font color="blue">'''RBS-luxI-RBS-lasI-T-Plas'''</font> | ||
|<font color="blue">'''pSB1A2'''</font> | |<font color="blue">'''pSB1A2'''</font> | ||
| Line 287: | Line 287: | ||
*<font color="green">'''Green'''</font>-coloured parts have been succesfully sequenced. | *<font color="green">'''Green'''</font>-coloured parts have been succesfully sequenced. | ||
*<font color="blue">'''Blue'''</font>-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct. | *<font color="blue">'''Blue'''</font>-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct. | ||
| - | *<font color="red">'''Red'''</font>-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts | + | *<font color="red">'''Red'''</font>-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts seemed to work and so we decided to submit them. |
| - | *24, 25, 34, 35, 36 are | + | *24, 25, 34, 35, 36 are parts of our final Mux or Demux systems. |
*Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages! | *Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages! | ||
*Parts in <font color="gray">'''gray'''</font> were programmed, but they have not been assembled and so they haven't been submitted. | *Parts in <font color="gray">'''gray'''</font> were programmed, but they have not been assembled and so they haven't been submitted. | ||
Latest revision as of 14:36, 27 October 2008
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|
|---|---|---|---|---|
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|
Assembled Parts Notation
In notebook section we sometimes use abbreviations to name assembled parts. New parts' official name, notebook abbreviations and new parts description are reported in the following table:
| New Part | Notebook Name | Assembled Parts(code) | Assembled Parts(name) | Vector | Description |
|---|---|---|---|---|---|
| - | 01 | J23100-E0240 | Pcon-GFP_protein_generator | J61002 | Test |
| K081005 | 02 | J23100-B0030 | Pcon-RBS | pSB1A2 | Intermediate |
| K081006 | 03 | R0051-B0030 | Plambda-RBS | pSB1A2 | Intermediate |
| S04119 | 04 | B0030-E0040 | RBS-GFP | pSB1A2 | Intermediate |
| K081007 | 05 | B0030-C0051 | RBS-cI | pSB1A2 | Intermediate |
| S04120 | 06 | J23100-E1010 | RBS-RFP | pSB1A2 | Intermediate |
| K081008 | 07 | B0030-C0061 | RBS-luxI | pSB1A2 | Intermediate |
| K081009 | 08 | B0030-C0078 | RBS-lasI | pSB1A2 | Intermediate |
| - | 09 | K081005-C0012 | Pcon-RBS-lacI | pSB1A2 | Intermediate |
| K081010 | 10 | K081005-C0040 | Pcon-RBS-tetR | pSB1A2 | Intermediate |
| - | 11 | K081005-I15010 | Pcon-RBS-cph8 | pSB1A2 | Intermediate |
| K081011 | 12 | K081006-C0062 | Plambda-RBS-luxR | pSB1A2 | Intermediate |
| K081012 | 13 | S04119-B1006 | RBS-GFP-T | pSB1AK3 | GFP protein generator with 39bp artificial terminator |
| K081013 | 14 | K081007-B0030 | RBS-cI-RBS | pSB1A2 | Intermediate |
| K081014 | 15 | S04120-B1006 | RBS-RFP-T | pSB1AK3 | GFP protein generator with 39bp artificial terminator |
| K081015 | 16 | K081008-B1006 | RBS-luxI-T | pSB1AK3 | luxI protein generator RCT |
| K081016 | 17 | K081009-B1006 | RBS-lasI-T | pSB1AK3 | lasI protein generator RCT |
| K081017 | 18 | B0030-I15009 | RBS-pcyA | pSB1A2 | Intermediate |
| - | 19 | (K081005-C0012)-B1006 | Pcon-RBS-lacI-T | pSB1AK3 | lacI protein generator PRCT |
| K081018 | 20 | K081010-B1006 | Pcon-RBS-tetR-T | pSB1AK3 | tetR protein generator PCRT |
| - | 21 | (K081005-I15010)-B0030 | Pcon-RBS-cph8-RBS | ? | Intermediate |
| K081019 | 22 | K081011-B1006 | Plambda-RBS-luxR-T | pSB1AK3 | Plambda regulated luxR protein generator PRCT |
| K081020 | 23 | K081013-C0079 | RBS-cI-RBS-lasR | pSB1A2 | Intermediate |
| K081000 | 24 | K081015-R0062 | RBS-luxI-T-Plux | pSB1A2 | Mux - Channel 0 |
| K081001 | 25 | K081016-R0079 | RBS-lasI-T-Plas | pSB1A2 | Mux - Channel 1 |
| K081021 | 26 | K081017-B1006 | RBS-pcyA-T | pSB1AK3 | pcyA protein generator RCT |
| - | 27 | (K081005-C0012-B1006)-R0010 | Pcon-RBS-lacI-T-Plac | pSB1AK3 | lacI composite part: PRCT-Plac |
| - | 28 | K081018-R0040 | Pcon-RBS-tetR-T-Ptet | pSB1A2 | tetR composite part: PRCT-Ptet |
| - | 29 | (K081005-I15010-B0030)-I15008 | Pcon-RBS-cph8-RBS-ho1 | ? | Intermediate |
| K081022 | 30 | K081019-R0062 | Plambda-RBS-luxR-T-Plux | pSB1A2 | luxR composite part: Plambda regulated PRCT-Plux |
| K081023 | 31 | K081020-B1006 | RBS-cI-RBS-lasR-T | pSB1AK3 | cI-lasR protein generator RCRCT |
| K081024 | 32 | K081021-R0082 | RBS-pcyA-T-Pomp | pSB1A2 | pcyA composite part: RCT-Pomp |
| - | 33 | (K081005-I15010-B0030-I15008)-K081024 | Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp | ? | Light responsive system under constitutive promoter: PRCRCRCT-Pomp |
| K081004 | 34 | K081023-K081022 | RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux | pSB1AK3 | Demux - Selector |
| K081002 | 35 | K081023-K081019 | RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T | pSB1AK3 | Mux - Selector |
| K081003 | 36 | K081008-K081001 | RBS-luxI-RBS-lasI-T-Plas | pSB1A2 | Demux - Input |
- 01 part has not been submitted to the registry because it was a test assembly.
- Red-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
- Green-coloured parts have been succesfully sequenced.
- Blue-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct.
- Red-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts seemed to work and so we decided to submit them.
- 24, 25, 34, 35, 36 are parts of our final Mux or Demux systems.
- Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages!
- Parts in gray were programmed, but they have not been assembled and so they haven't been submitted.
