"
Team:Warsaw/Calendar-Main/13 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| (8 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<html> | <html> | ||
| - | <h3>Checking for presence of A on the cell membrane< | + | <h3>Checking for presence of protein A on the cell membrane</h3> |
| - | Piotr</ | + | <h4>Piotr</h4> |
<p><ol> | <p><ol> | ||
| - | <li>Pouring bacteria by drops onto nitrocellulose</li> | + | <li>Pouring bacteria by drops onto nitrocellulose.</li> |
| - | <li>Drying</li> | + | <li>Drying.</li> |
| - | <li>Blocking</li> | + | <li>Blocking.</li> |
| - | <li>Anti-A antibody binding</li> | + | <li>Anti-A antibody binding.</li> |
| - | <li>Washing</li> | + | <li>Washing.</li> |
| - | <li>Anti-rabbit antibody binding</li> | + | <li>Anti-rabbit antibody binding.</li> |
| - | <li>Developing with BCIP and NBT</li> | + | <li>Developing with BCIP and NBT (see <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008#fig1">Fig. 1.</a>).</li> |
</ol> | </ol> | ||
| - | </p> | + | </p><a name="fig1"><img src="https://static.igem.org/mediawiki/2008/e/ec/Blot_OmpA_WAW.jpg" width=150/></a> |
| + | <var><b>Fig. 1.</b>Protein A detection on the surface of bacteria.<br> | ||
| + | 1 - <i>E. coli</i> expressing OmpA_Omega_A_Alpha,<br> | ||
| + | 2 - <i>E. coli</i> expressing OmpA_Z_Omega (antibodies binds to protein Z probably due to similarity of protein A and Z),<br> | ||
| + | 3 - <i>E. coli</i> expressing OmpA_A_Alpha,<br> | ||
| + | 4 - <i>E. coli</i> carrying OmpA_Omega_A_Alpha without inducer,<br> | ||
| + | 5 - <i>E. coli</i> expressing OmpA_A_Omega,<br> | ||
| + | 6 - <i>E. coli</i> Top10 without plasmid.</var> | ||
| + | |||
| + | <br><br> | ||
| + | |||
| + | <h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4> | ||
| + | <p> Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> plasmids to LB with kanamycin.</p> | ||
| + | |||
| - | |||
</html> | </html> | ||
Latest revision as of 18:04, 27 October 2008
 |
|
||||||||
 |
|
||||||||
Checking for presence of protein A on the cell membranePiotr
Fig. 1.Protein A detection on the surface of bacteria.1 - E. coli expressing OmpA_Omega_A_Alpha, 2 - E. coli expressing OmpA_Z_Omega (antibodies binds to protein Z probably due to similarity of protein A and Z), 3 - E. coli expressing OmpA_A_Alpha, 4 - E. coli carrying OmpA_Omega_A_Alpha without inducer, 5 - E. coli expressing OmpA_A_Omega, 6 - E. coli Top10 without plasmid. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.Inoculation of pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega plasmids to LB with kanamycin.
|
|
