Team:Warsaw/Calendar-Main/20 October 2008

From 2008.igem.org

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<h3>Preparation of OmpA-linker-alpha-linker</h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac</a></h3>
<h4>Michał K., Piotr</h4>
<h4>Michał K., Piotr</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
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<li> Tranformants planting on LB with kanamycin. </li>
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<li> Tranformants plating on LB with kanamycin. </li>
</ol>
</ol>

Revision as of 20:54, 27 October 2008

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Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AraC+pBAD+AID to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac

Michał K., Piotr

  1. Digest of pLac_OmpA_alpha and OmpA-linker (BBa_K103006) with EcoRI and SacI (BamHI buffer).
  2. Gel electrophoresis and gel-out of proper band 500 bp for OmpA-linker (BBa_K103006) lane and 3000 bp for pLac_OmpA_alpha lane.
  3. Ligation of isolated DNA fragments.
  4. Transformation of TOP10 with above ligation.
  5. Tranformants plating on LB with kanamycin.

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