Team:Warsaw/Calendar-Main/22 October 2008

From 2008.igem.org

(Difference between revisions)
Line 15: Line 15:
-
 
+
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a></h3>
-
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker</a></h3>
+
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol>
<ol>

Revision as of 21:00, 27 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AraC+pBAD+AID.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - OmpA_alpha_.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008"