Brown: Team Resistance/26 October 2008

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(New page: {{BNBK}} ===26 October 2008=== *Made new culture of the PVJ4 transformation so that a miniprep can be done. *New culture of gene S105 part made. *Will run a gel of the S105 gene restri...)
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{{BNBK}}
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===26 October 2008===
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==26 October 2008==
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====Biobricking work====
*Made new culture of the PVJ4 transformation so that a miniprep can be done.   
*Made new culture of the PVJ4 transformation so that a miniprep can be done.   
*New culture of gene S105 part made.
*New culture of gene S105 part made.
-
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part.
+
Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
 +
*pBAD prmoter
 +
*IPTG inducible promoter
 +
*Mercury inducible promoter
 +
*Pu inducible promoter (xylene induced)
 +
*T7 promoter, with activity modified by change in temperature
 +
 
 +
Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)
 +
 
 +
*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector.  We should have seen two bands but we only saw one.
 +
 
 +
====Conductivity Tests====
 +
 
 +
*PVJ4 Colony 2 and 4 plus Controls.  10ml cultures.  420 ul of 50% Arabinose solution will be added.  Conductance measurements will be taken before and after lysis has occurred. 
 +
*Throughout the test, Optical Density measurements will be taken.

Latest revision as of 21:52, 27 October 2008


Toxipop lab notebook.png

26 October 2008

Biobricking work

  • Made new culture of the PVJ4 transformation so that a miniprep can be done.
  • New culture of gene S105 part made.

Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:

  • pBAD prmoter
  • IPTG inducible promoter
  • Mercury inducible promoter
  • Pu inducible promoter (xylene induced)
  • T7 promoter, with activity modified by change in temperature

Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)

  • Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.

Conductivity Tests

  • PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
  • Throughout the test, Optical Density measurements will be taken.
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