Brown: Team Resistance/26 October 2008

(Difference between revisions)

Latest revision as of 21:52, 27 October 2008

Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:

Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)

Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.

PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
Throughout the test, Optical Density measurements will be taken.

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 {{BNBK}}
-===26 October 2008===
+==26 October 2008==
+====Biobricking work====
 *Made new culture of the PVJ4 transformation so that a miniprep can be done.
 *New culture of gene S105 part made.
-*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector.  We should have seen two bands but we only saw one.
+Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
+*pBAD prmoter
+*IPTG inducible promoter
+*Mercury inducible promoter
+*Pu inducible promoter (xylene induced)
+*T7 promoter, with activity modified by change in temperature
+Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)
+*Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector.  We should have seen two bands but we only saw one.
 ====Conductivity Tests====