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Team:Johns Hopkins/Protocols
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== Protocols == | == Protocols == | ||
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[[Media:JHU_0708_Jaime-protocols.pdf|PCR Protocols from Jaime]]<br> | [[Media:JHU_0708_Jaime-protocols.pdf|PCR Protocols from Jaime]]<br> | ||
| - | [[Media: | + | [[Media:JHU_0708_IGEM_Ligation_and_Transformation.pdf|Ligation/Cloning and Transformation]]<br> |
[[Media:JHU_0708_Colony_Screening_PCR.pdf|Moodle Colony Screening PCR]]<br> | [[Media:JHU_0708_Colony_Screening_PCR.pdf|Moodle Colony Screening PCR]]<br> | ||
| + | [[Media:JHU_0708_Plasmid_Miniprep_Protocol.pdf|Plasmid Miniprep]] <br> | ||
| + | [[Media:CMBD.pdf|Checking Minipreps to Prepare for Sequencing]] <br> | ||
| + | [[Media:SSS.pdf|Sequence Sample Submission]] <br> | ||
| + | [[Media:JHU_Glycerol_Stocks.pdf|Making Glycerol Stocks]]<br> | ||
| + | [[Media:JHU_Moving_Insert_to_iGEM_Vector.pdf| Moving Insert to iGEM Vector]]<br> | ||
== Papers == | == Papers == | ||
=== Important Papers === | === Important Papers === | ||
| - | + | <html> | |
| + | <a href="http://www.jbioleng.org/content/2/1/5">Reshma P Shetty, Drew Endy, and Thomas F Knight Jr; <i>Engineering BioBrick vectors from BioBrick parts</i></a><br> | ||
| + | </html> | ||
| - | [[Media: | + | [[Media:JHU_0708_paper_Aregularoryheirarchy.pdf| Herskowitz I. <i> A regulatory hierarchy for cell specialization in yeast. </i> (REVIEW.) Nature. 342:749-757 (1989)<br>]] |
| - | [[Media: | + | [[Media:JHU_0708_paper_MolecularMechanismofcell-typedeterminationinbuddingyeast.pdf|Johnson AD. <i> Molecular mechanisms of cell-type determination in budding yeast. </i>(REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)<br>]] |
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| + | [[Media:JHU_0708_paper_The_N-end_Rule_Pathway_as_a_Nitric_Oxide_Sensor_Controlling_the_Levels_of_Multiple_Regulators.pdf| Hu R-G <I>et al.</I> <I>The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators.</I> Nature. 437:981-986 (2005)<br>]] | ||
| - | [[Media:JHU_0708_paper_TheN-endRule.pdf| | + | [[Media:JHU_0708_paper_TheN-endRule.pdf|Varshavsky A. <I>The N-end Rule pathway of protein degradation. (REVIEW.)</I> Genes to Cells. 2:13-28 (1997)<br>]] |
| - | [[Media:JHU_0708_paper_Optimizedcassettes.pdf| | + | [[Media:JHU_0708_paper_Optimizedcassettes.pdf| Sheff MA & Thorn KS. <I>Optimized cassettes for fluorescent protein tagging in</I> Saccharomyces cerevisiae. Yeast. 21:661-670 (2004)<br>]] |
| - | [[Media:JHU_0708_paper_Awalk.pdf| | + | [[Media:JHU_0708_paper_Awalk.pdf| Bardwell L. <I>A walk-through of the yeast mating pheromone response pathway. (REVIEW.)</I> Peptides. 25:1465-1476 (2004)<br>]] |
=== Yeast Vector Papers === | === Yeast Vector Papers === | ||
| - | [[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf| | + | [[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf|Broach JR. <I>Transformation in yeast: development of a hybrid cloning vector and isolation of the </I>CAN1<I> gene.</I> Gene. 8(1):121-133 (1979)<br>]] |
| - | [[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf| | + | [[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf|Stearns, T. <I>Manipulating yeast genome using plasmid vectors. (REVIEW.)</I> Methods in enzymology. 185:280-297 (1990)<br>]] |
| - | Stearns, T. <I>Manipulating yeast genome using plasmid vectors. (REVIEW.)</I> Methods in enzymology. 185:280-297 (1990)<br> | + | |
| - | [[Media:JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf| | + | [[Media:JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf| Hill JE <I>et al.</I> <I>Yeast/</I>E. coli<I> Shuttle Vectors with Multiple Unique Restriction Sites.</I> Yeast. 2:163-167 (1986)<br>]] |
| - | [[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf| | + | [[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf|Sikorski RS. <I>A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in </I>Saccharomyces cerevisiae. Genetics. 122:19-27 (1989)<br>]] |
| - | Sikorski RS. <I>A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in </I>Saccharomyces cerevisiae. Genetics. 122:19-27 (1989)<br> | + | |
| - | [[Media:JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf| | + | [[Media:JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf| Mumberg D <I>et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.</I> Gene. 156:119-122 (1995)<br>]] |
=== Other === | === Other === | ||
Latest revision as of 22:59, 27 October 2008
Contents |
Protocols
PCR Protocols from Jaime
Ligation/Cloning and Transformation
Moodle Colony Screening PCR
Plasmid Miniprep
Checking Minipreps to Prepare for Sequencing
Sequence Sample Submission
Making Glycerol Stocks
Moving Insert to iGEM Vector
Papers
Important Papers
Reshma P Shetty, Drew Endy, and Thomas F Knight Jr; Engineering BioBrick vectors from BioBrick parts
Yeast Vector Papers
Other
JHU_0708_paper_TransfofYeast.pdf
JHU_0708_paper_Three-prongedgenomicanalysis.pdf
JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf
JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf
JHU_0708_paper_GlobalTranscriptionRegulators.pdf
JHU_0708_paper_ForeignGeneExpression.pdf
JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf
JHU_0708_paper_BentDNA.pdf
JHU_0708_paper_Aregularoryheirarchy.pdf
