Team:Warsaw/Calendar-Main/20 October 2008

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<li> Tranformants plating on LB with kanamycin. </li>
<li> Tranformants plating on LB with kanamycin. </li>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/5/58/Go_20_10_2008.jpg"></a>
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<var><b>Fig. 12.</b>Go_20_10_2008.jpg </var>
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Revision as of 23:44, 27 October 2008

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Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K., Piotr

  1. Digest of pLac_OmpA_alpha and OmpA-linker (BBa_K103006) with EcoRI and SacI (BamHI buffer).
  2. Gel electrophoresis and gel-out of proper band 500 bp for OmpA-linker (BBa_K103006) lane and 3000 bp for pLac_OmpA_alpha lane.
  3. Ligation of isolated DNA fragments.
  4. Transformation of TOP10 with above ligation.
  5. Tranformants plating on LB with kanamycin.
    6. Fig. 12.Go_20_10_2008.jpg

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