Team:NTU-Singapore/Notebook/3 June 2008

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(New page: ==Wednesday== *Chin Chong (~~) **Confirmed the orders for the oligo/primers designs to be purchase from 1st Base **List of things to purchase modified **Updated time-line for ordering che...)
 
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==Wednesday==
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*Chin Chong (~~)
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=Tuesday 3 June=
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<div class="quote" style="margin:20px;">
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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===Chin Chong (~~)===
**Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
**Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
**List of things to purchase modified
**List of things to purchase modified
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**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
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*ZhenFu
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===ZhenFu===
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Modified model to due to more estimation of parameters
**Modified model to due to more estimation of parameters
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*Darius
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===Darius===
**Printed and pasted the Grand master plan of our project on the wall
**Printed and pasted the Grand master plan of our project on the wall
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
   
   
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*Min Lin
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===Min Lin===
**Send out enquiries for potential sponsors
**Send out enquiries for potential sponsors
**Waiting aproval for Proposal
**Waiting aproval for Proposal
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*Hung
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===Hung===
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Processing and storage of amplified DNA plasmids
**Processing and storage of amplified DNA plasmids
**Updated Wiki website
**Updated Wiki website
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*Choon Kit
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===Choon Kit===
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
**Processing and storage of amplified DNA plasmids
**Processing and storage of amplified DNA plasmids
**Document laboratory reports for 02 Jun 2008
**Document laboratory reports for 02 Jun 2008
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=Lab details=
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===Personnel===
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*Hung,Choon Kit,Zhen Fu:
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**Morning:
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**#Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
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**##2 tubes with LBA contained no cell pellets at all as expected.
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**##2 tubes with LB without Amp. had cell pellets at the bottom. We then poured out the liquid and the tubes at -20 oC fridge for future use.
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**#Take out from the incubator 5 agar plates. All had cell colonies growing on them except for LBA BW-W3110 plate. We stored them at 4 oC fridge.
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**Afternoon:
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**#Inoculation to obtain 4 cryotubes:
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**##2 containing LBA with BBa_B0015 plasmid cells
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**##2 containing LBA with LacI-GFP plasmid cells
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<br>Incubate 4 tubes overnight
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</div>

Latest revision as of 00:28, 28 October 2008

Contents

Tuesday 3 June

Chin Chong (~~)

    • Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
    • List of things to purchase modified
    • Updated time-line for ordering chemicals and consumables
    • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

ZhenFu

    • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
    • Modified model to due to more estimation of parameters

Darius

    • Printed and pasted the Grand master plan of our project on the wall
    • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

Min Lin

    • Send out enquiries for potential sponsors
    • Waiting aproval for Proposal

Hung

    • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
    • Processing and storage of amplified DNA plasmids
    • Updated Wiki website

Choon Kit

    • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
    • Processing and storage of amplified DNA plasmids
    • Document laboratory reports for 02 Jun 2008

Lab details

Personnel

  • Hung,Choon Kit,Zhen Fu:
    • Morning:
      1. Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
        1. 2 tubes with LBA contained no cell pellets at all as expected.
        2. 2 tubes with LB without Amp. had cell pellets at the bottom. We then poured out the liquid and the tubes at -20 oC fridge for future use.
      2. Take out from the incubator 5 agar plates. All had cell colonies growing on them except for LBA BW-W3110 plate. We stored them at 4 oC fridge.
    • Afternoon:
      1. Inoculation to obtain 4 cryotubes:
        1. 2 containing LBA with BBa_B0015 plasmid cells
        2. 2 containing LBA with LacI-GFP plasmid cells


Incubate 4 tubes overnight

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