Team:NTU-Singapore/Notebook/4 June 2008

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=Wednesday 4 June=
=Wednesday 4 June=
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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==Lab details:==
==Lab details:==
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===Personnel===
Choon Kit, Hung, Darius:
Choon Kit, Hung, Darius:
*Morning:
*Morning:
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**# 2 for terminators
**# 2 for terminators
**# 2 for LacI-GFP
**# 2 for LacI-GFP
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**# 1 for CoE7 plasmid from BW strain (from -80 oC fridge)
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**# 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
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**# 1 for CoE7 plasmid from W3110 strain (from -80 oC fridge)
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**# 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)
*Afternoon:
*Afternoon:
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**Prepare LB broth  
**Prepare LB broth  
**Prepare 17x LBA agar plates
**Prepare 17x LBA agar plates
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Latest revision as of 00:28, 28 October 2008

Wednesday 4 June

Lab details:

Personnel

Choon Kit, Hung, Darius:

  • Morning:
    • Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
    • Prepare 6 digestion solutions for gel electrophoresis in the afternoon:
      1. 2 for terminators
      2. 2 for LacI-GFP
      3. 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
      4. 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)
  • Afternoon:
    • Amplification for the following plasmids (from Registry):
      1. GFP generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]
      2. RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032] and RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
      3. T7 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005]
    • Run gel for morning samples.
    • Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
    • Prepare LB broth
    • Prepare 17x LBA agar plates
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