Team:NTU-Singapore/Notebook/20 June 2008

From 2008.igem.org

(Difference between revisions)
(New page: =Friday 20 June= ==Morning:== *Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit. ==Afternoon:== *Transformation and cell cloning 4 empty plasmids from ...)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
 +
<html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html>
 +
 +
<div id="header">{{User:Greenbear/sandbox/header}}</div>
 +
 +
<div id="maincontent" style="margin-top:130px;">
 +
<html>
 +
<div id="arrow">
 +
<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
 +
"
 +
    alt="Back to Notebook"
 +
    title="Back to Notebook">
 +
</a>
 +
</div>
 +
</html>
 +
 +
=Friday 20 June=
=Friday 20 June=
 +
<div class="quote" style="margin:20px;">
 +
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
 +
|
==Morning:==
==Morning:==
*Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit.
*Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit.
Line 8: Line 28:
**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]
**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]
**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3]
**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3]
 +
*Transformation and cloning of LacI-GFP into LuxS knockout bacteria.
*Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells.
*Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells.
 +
==Evening:==
==Evening:==
*Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples:
*Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples:
Line 15: Line 37:
**T7ptag Insert - Empty plasmid vector (from GFP)
**T7ptag Insert - Empty plasmid vector (from GFP)
**GFP insert - pFE vector (for characterization of Fe promoter)
**GFP insert - pFE vector (for characterization of Fe promoter)
 +
</div>

Latest revision as of 00:36, 28 October 2008


Contents

Friday 20 June

Morning:

  • Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit.

Afternoon:

  • Transformation and cell cloning 4 empty plasmids from Biobrick registry:
    • [http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3]
    • [http://partsregistry.org/wiki/index.php?title=Part:pSB1AC3 pSB1AC3]
    • [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]
    • [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3]
  • Transformation and cloning of LacI-GFP into LuxS knockout bacteria.
  • Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells.

Evening:

  • Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples:
    • E7 Insert - Empty plasmid vector (from GFP)
    • SupD Insert - Empty plasmid vector (from GFP)
    • T7ptag Insert - Empty plasmid vector (from GFP)
    • GFP insert - pFE vector (for characterization of Fe promoter)
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/20_June_2008"