Team:University of Lethbridge/Notebook/Project3October

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===October 21 2008===
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'''Andrew'''
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Transformation of +18 rpsA TIR DNA into pGEM TEZ to make enough DNA to be restricted/ligated into biobrick format. (psB1A2)
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50 uL og xgal added to LB + amp plates to screen for blue and white colonies.
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===October 22 2008===
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'''Andrew'''
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Transformation into pGEM TEZ successful, many white and some blue colonie on both plates.
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Picked 3 colonies from each plate to grow in 5mL LB + amp for a pDNA prep.
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===October 23 2008===
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'''Andrew'''
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pDNA prep of 6 culture tubes using a kit

Revision as of 01:02, 28 October 2008

Back to The University of Lethbridge Main Notebook




October 21 2008

Andrew

Transformation of +18 rpsA TIR DNA into pGEM TEZ to make enough DNA to be restricted/ligated into biobrick format. (psB1A2)

50 uL og xgal added to LB + amp plates to screen for blue and white colonies.



October 22 2008

Andrew

Transformation into pGEM TEZ successful, many white and some blue colonie on both plates.

Picked 3 colonies from each plate to grow in 5mL LB + amp for a pDNA prep.



October 23 2008

Andrew

pDNA prep of 6 culture tubes using a kit

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