USTC/1 August 2008
From 2008.igem.org
(Difference between revisions)
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*digest the biobrick BBa_B0034/32/30 with SpeI and pstI. | *digest the biobrick BBa_B0034/32/30 with SpeI and pstI. | ||
*digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI. | *digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI. | ||
- | *run gel and | + | *run gel and purify fragments from agarose gels |
- | *ligate B0034 + C0070-B0015,B0032+C0070,B0032+C0070. | + | *ligate B0034 + C0070-B0015,B0032 + C0070,B0032 + C0070. |
Latest revision as of 01:22, 28 October 2008
Experiments
- digest the biobrick BBa_B0034/32/30 with SpeI and pstI.
- digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI.
- run gel and purify fragments from agarose gels
- ligate B0034 + C0070-B0015,B0032 + C0070,B0032 + C0070.