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| - | [ | + | [[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] |
| + | |||
| + | ===September 30, 2008=== | ||
| + | |||
| + | '''Andrew''' | ||
| + | |||
| + | PCR of genomic + rpsA DNA with two different sets of primers | ||
| + | |||
| + | Reaction Conditions: | ||
| + | - 1.0 uL Template DNA | ||
| + | - 5.0 uL 10x Buffer | ||
| + | - 2.0 uL 10 mM dNTPs | ||
| + | - 1.0 uL Forward Primer | ||
| + | - 1.0 uL Reverse Primer | ||
| + | - 0.5 uL Econo taq polymerase | ||
| + | - 39.5 uL Optima H2O | ||
| + | |||
| + | PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA | ||
| + | |||
| + | |||
| + | Cycling Conditions: | ||
| + | 1. 94 C 2 min | ||
| + | 2. a. 94 C 15 sec | ||
| + | b. 47 C 15 sec | ||
| + | c. 72 C 15 sec | ||
| + | 3. Repeat step 2 for 25 cycles | ||
| + | 4.72 C 5 min | ||
| + | |||
| + | [[Image:rpsA PCR.jpg|250px]] | ||
Latest revision as of 01:36, 28 October 2008
Back to The University of Lethbridge Main Notebook
September 30, 2008
Andrew
PCR of genomic + rpsA DNA with two different sets of primers
Reaction Conditions:
- 1.0 uL Template DNA - 5.0 uL 10x Buffer - 2.0 uL 10 mM dNTPs - 1.0 uL Forward Primer - 1.0 uL Reverse Primer - 0.5 uL Econo taq polymerase - 39.5 uL Optima H2O
PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA
Cycling Conditions:
1. 94 C 2 min 2. a. 94 C 15 sec b. 47 C 15 sec c. 72 C 15 sec 3. Repeat step 2 for 25 cycles 4.72 C 5 min
