Team:University of Lethbridge/Notebook/Project3September

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===September 30, 2008===
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'''Andrew'''
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PCR of genomic + rpsA DNA with two different sets of primers
 +
 +
Reaction Conditions:
 +
  - 1.0 uL Template DNA
 +
  - 5.0 uL 10x Buffer
 +
  - 2.0 uL 10 mM dNTPs
 +
  - 1.0 uL Forward Primer
 +
  - 1.0 uL Reverse Primer
 +
  - 0.5 uL Econo taq polymerase
 +
  - 39.5 uL Optima H2O
 +
 +
PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA
 +
 +
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Cycling Conditions:
 +
1. 94 C 2 min
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2. a. 94 C 15 sec
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    b. 47 C 15 sec
 +
    c. 72 C 15 sec
 +
3. Repeat step 2 for 25 cycles
 +
4.72 C 5 min
 +
 +
[[Image:rpsA PCR.jpg|250px]]

Latest revision as of 01:36, 28 October 2008

Back to The University of Lethbridge Main Notebook

September 30, 2008

Andrew

PCR of genomic + rpsA DNA with two different sets of primers

Reaction Conditions: 

 - 1.0 uL Template DNA
 - 5.0 uL 10x Buffer
 - 2.0 uL 10 mM dNTPs
 - 1.0 uL Forward Primer
 - 1.0 uL Reverse Primer
 - 0.5 uL Econo taq polymerase
 - 39.5 uL Optima H2O

PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA


Cycling Conditions: 

1. 94 C 2 min
2. a. 94 C 15 sec
   b. 47 C 15 sec
   c. 72 C 15 sec 
3. Repeat step 2 for 25 cycles
4.72 C 5 min

RpsA PCR.jpg

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