Team:NTU-Singapore/Notebook/5 July 2008

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(New page: =Saturday 5 July= *Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. *Lu Chao: minipreps for LacI-RBS 34. *...)
 
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=Saturday 5 July=
=Saturday 5 July=
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*Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony.
*Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony.
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*Lu Chao: minipreps for LacI-RBS 34.
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===Lu Chao===
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*Hung, Lu Chao:
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: minipreps for LacI-RBS 34.
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===Hung, Lu Chao===
**Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results.
**Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results.
**Prepare digestion mixtures for gel confirmation.
**Prepare digestion mixtures for gel confirmation.
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*Darius:Gel electrophoresis to check if there are LacI-RBS 34 plasmids.<br>Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band:
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===Darius===
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:Gel electrophoresis to check if there are LacI-RBS 34 plasmids.<br>Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band:
**There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!)
**There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!)
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**There is 2 samples that have both 250 bp band and 200 bp band, which can be interpreted as GFP (due to self-ligation of GFP).
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**There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS).
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**The rest only have the RBS band.
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Latest revision as of 02:24, 28 October 2008


Contents

Saturday 5 July

  • Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony.

Lu Chao

minipreps for LacI-RBS 34.

Hung, Lu Chao

    • Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results.
    • Prepare digestion mixtures for gel confirmation.

Darius

Gel electrophoresis to check if there are LacI-RBS 34 plasmids.
Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band:
    • There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!)
    • There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS).
    • The rest only have the RBS band.
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