Team:NTU-Singapore/Notebook/16 July 2008
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=Wednesday 16 July= | =Wednesday 16 July= | ||
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*Morning: We carried out several digestion: | *Morning: We carried out several digestion: | ||
**GFP with E/P in buffer 2 for 1 hour; in buffer 2 for 2 hours; and in EcoRI buffer for 2 hours (this aims to compare the efficiency of buffer 2 with EcoRIbuffer for E/P double digestion; as well as the optimal digestion time). | **GFP with E/P in buffer 2 for 1 hour; in buffer 2 for 2 hours; and in EcoRI buffer for 2 hours (this aims to compare the efficiency of buffer 2 with EcoRIbuffer for E/P double digestion; as well as the optimal digestion time). | ||
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*1650: gel extraction. After this, the digested DNAs were put in -20 fridge for ligation on Thursday. | *1650: gel extraction. After this, the digested DNAs were put in -20 fridge for ligation on Thursday. | ||
*1520: digestion for gel check of 12 samples of Fe-GFP inoculated yesterday. However,no colony was found correct. | *1520: digestion for gel check of 12 samples of Fe-GFP inoculated yesterday. However,no colony was found correct. | ||
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Latest revision as of 02:30, 28 October 2008
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Wednesday 16 July
DNA: 1000ng (around 5ul) Enzyme1: 1 ul Enzyme2: 1 ul Buffer: 5 ul BSA: 0.5 ul H2O: 37.5 ul Total: 50
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