Team:NTU-Singapore/Notebook/19 August 2008
From 2008.igem.org
(Difference between revisions)
m |
Lalala8585 (Talk | contribs) |
||
(One intermediate revision not shown) | |||
Line 1: | Line 1: | ||
+ | <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> | ||
+ | |||
+ | <div id="header">{{User:Greenbear/sandbox/header}}</div> | ||
+ | |||
+ | <div id="maincontent" style="margin-top:130px;"> | ||
+ | <html> | ||
+ | <div id="arrow"> | ||
+ | <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook"> | ||
+ | <img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png | ||
+ | " | ||
+ | alt="Back to Notebook" | ||
+ | title="Back to Notebook"> | ||
+ | </a> | ||
+ | </div> | ||
+ | </html> | ||
+ | |||
+ | |||
=Tuesday 19 August= | =Tuesday 19 August= | ||
+ | <div class="quote" style="margin:20px;"> | ||
+ | {|border="1" style="background-color:#ffffcc;" cellpadding="20" | ||
+ | | | ||
*800-930: Lu Chao: minipreps for 5xE7-term colonies and 7xlysis-term colonies. Prepare 2% gel. | *800-930: Lu Chao: minipreps for 5xE7-term colonies and 7xlysis-term colonies. Prepare 2% gel. | ||
*1330-1400: Hung: digestion of the above plasmids with EcoRI and PstI for checking. Incubate for 2 hours. | *1330-1400: Hung: digestion of the above plasmids with EcoRI and PstI for checking. Incubate for 2 hours. | ||
Line 7: | Line 27: | ||
WELL! So we only have 2 more ligations to do: LacI-RBS with E7-Term and LsrA-RBS with Lysis-Term ! | WELL! So we only have 2 more ligations to do: LacI-RBS with E7-Term and LsrA-RBS with Lysis-Term ! | ||
[[Image:NTU_gel_19_08.JPG|500px|center|thumb|from left to right: 1kb ladder, 5xE7-Term, 100bp ladder, 7xLysis-Term]] | [[Image:NTU_gel_19_08.JPG|500px|center|thumb|from left to right: 1kb ladder, 5xE7-Term, 100bp ladder, 7xLysis-Term]] | ||
+ | </div> |
Latest revision as of 02:36, 28 October 2008
|
Tuesday 19 August
WELL! So we only have 2 more ligations to do: LacI-RBS with E7-Term and LsrA-RBS with Lysis-Term ! |