Team:Warsaw/Calendar-Main/5 May 2008

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Latest revision as of 10:40, 28 October 2008


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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Piotr

Setup of overnight culture E. coli TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA.

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+<html>
-<p>Piotr: Setup of overnight culture <i>E. coli</i> TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA<br>
+<h3> Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a> </h3>
-<br>
+<h4>Piotr</h4>
-'''TOP10 (Invitrogen)'''<br>
-<br>
-F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- <br>
-<br>
-'''Rosetta(DE3)pLysS'''<br>
-<br>
-F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)<br>
-</p>
+<p>
+Setup of overnight culture <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> (LB broth) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA.<br>
+</p>
+</html>
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