Team:Warsaw/Calendar-Main/22 October 2008

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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/4/44/Traw_22_10_2008.jpg"></a>
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<var><b>Fig. 28.</b>traw_22_10_2008.jpg </var>
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<var><b>Fig.1.</b>Control EcoRI/PstI digests of isolated plasmids.
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1. Marker<br>
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2-9. EcoRI/PstI digests of isolated plasmids<br></var>

Revision as of 13:16, 28 October 2008

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Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
Fig.1.Control EcoRI/PstI digests of isolated plasmids. 1. Marker
2-9. EcoRI/PstI digests of isolated plasmids

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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