Team:Warsaw/Calendar-Main/30 June 2008

From 2008.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
<html>
<html>
-
<h3>Cloning alpha-A and omega-A fusions on pKS</h3>
+
 
-
<h4>Michał L., Ewa, Marcin:</h4>
+
<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
 +
<h4>Piotr, Weronika</h4>
 +
<ol>
 +
<li>There were blue and white colonies but no red or green in UV.</li>
 +
 
 +
<li>Inoculation of liquid LB medium + Amp with white colonies.</li>
 +
<li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>.</li>
 +
<li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator).
 +
 
 +
</li></ol>
 +
 
 +
 
 +
<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
 +
<h4>Michał L., Ewa, Marcin</h4>
<p>Inoculation of liquid LB medium with 6 transformant colonies.</p>
<p>Inoculation of liquid LB medium with 6 transformant colonies.</p>
</ol>
</ol>
-
<h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4>
+
<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3><h4>Paweł</h4>
-
<p> Inoculation of <i> E. coli</i> with pET15b plasmid on LB + ampicillin.</p>
+
<p> Inoculation of <i> E. coli</i> with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p>
</html>
</html>

Latest revision as of 14:03, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. There were blue and white colonies but no red or green in UV.
  2. Inoculation of liquid LB medium + Amp with white colonies.
  3. Inoculation of 10ml LB with E.coli TOP10 carrying pZC320.
  4. Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(GFP genetrator) and BBa_J04450 (RFP generator).

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Inoculation of E. coli with pET15b plasmid on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008"