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Team:Warsaw/Calendar-Main/30 June 2008
From 2008.igem.org
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<h4>Piotr, Weronika</h4> | <h4>Piotr, Weronika</h4> | ||
<ol> | <ol> | ||
| - | <li>There were blue and white colonies but no red or green in UV</li> | + | <li>There were blue and white colonies but no red or green in UV.</li> |
| - | <li>Inoculation of liquid LB medium + Amp with white colonies</li> | + | <li>Inoculation of liquid LB medium + Amp with white colonies.</li> |
| - | <li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a></li> | + | <li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>.</li> |
| - | <li>Inoculation of E.coli TOP10 carrying | + | <li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator). |
</li></ol> | </li></ol> | ||
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</ol> | </ol> | ||
| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3><h4>Paweł</h4> |
<p> Inoculation of <i> E. coli</i> with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p> | <p> Inoculation of <i> E. coli</i> with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p> | ||
Latest revision as of 14:03, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinInoculation of liquid LB medium with 6 transformant colonies. Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPawełInoculation of E. coli with pET15b plasmid on LB + ampicillin.
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