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Team:Warsaw/Calendar-Main/3 July 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Preparation of constructs with OmpA protein fusions'''<br> 1. Clean-up of overnight digestation <br> 2. Ligation of OmpA_alfa and pE...) |
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| + | <h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3> | ||
| + | <h4>Piotr, Weronika</h4> | ||
| + | <ol> | ||
| + | <li>There were blue and white colonies but no red or green fluorescence in UV.</li> | ||
| + | <li>Inoculation of liquid LB medium + Amp with white colonies.</li> | ||
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| + | <h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3> | ||
| + | <h4>Paweł</h4> | ||
| + | <p> | ||
| + | Colonies from each transformant strain from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p> | ||
| + | <h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | ||
| + | <h4>Michał L., Ewa, Marcin</h4> | ||
| + | <p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.</p> | ||
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Latest revision as of 14:07, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPawełColonies from each transformant strain from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin. Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinWe have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.
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