Team:Warsaw/Calendar-Main/3 July 2008

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Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

There were blue and white colonies but no red or green fluorescence in UV.
Inoculation of liquid LB medium + Amp with white colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Colonies from each transformant strain from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.

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+<html>
+<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
+<h4>Piotr, Weronika</h4>
+<ol>
+<li>There were blue and white colonies but no red or green fluorescence in UV.</li>
+<li>Inoculation of liquid LB medium + Amp with white colonies.</li>
-<p>'''Preparation of constructs with OmpA protein fusions'''<br>
+</li></ol>
-. Clean-up of overnight digestation <br>
-. Ligation of OmpA_alfa and pET15b <br>
-. Ligation of OmpA_omega and pET15b<br>
-. Transformation of E. coli TOP10 strain with these ligations <br>
-. Transformants plating on LB + ampicillin
-</p>
+<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3>
+<h4>Paweł</h4>
+<p>
+Colonies from each transformant strain from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p>
+<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
+<h4>Michał L., Ewa, Marcin</h4>
+<p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.</p>
+</html>
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