Team:Warsaw/Calendar-Main/3 July 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 14:07, 28 October 2008


	Attribution analyzer / Interactive list of topics

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

There were blue and white colonies but no red or green fluorescence in UV.
Inoculation of liquid LB medium + Amp with white colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Colonies from each transformant strain from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.

@@ Line 1: / Line 1: @@
 {{WarNotebook}}
 <!-- do not edit above me! -->
+<html>
-<h3>Preparation of constructs with OmpA protein fusions
+<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
-<br>Paweł</h3>
+<h4>Piotr, Weronika</h4>
-<ol><li>
+<ol>
-Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.</li></ol>
+<li>There were blue and white colonies but no red or green fluorescence in UV.</li>
-<html>
+<li>Inoculation of liquid LB medium + Amp with white colonies.</li>
-<h3>Cloning alpha-A and omega-A fusions on pKS</h3>
-<h4>Michał L., Ewa, Marcin:</h4>
+</li></ol>
-<p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p>
+<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3>
+<h4>Paweł</h4>
+<p>
+Colonies from each transformant strain from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p>
+<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
+<h4>Michał L., Ewa, Marcin</h4>
+<p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.</p>
 </html>