Team:Warsaw/Calendar-Main/3 July 2008

From 2008.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 3: Line 3:
<html>
<html>
-
<h3>Preparation of constructs with OmpA protein fusions</h3>
+
<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
 +
<h4>Piotr, Weronika</h4>
 +
<ol>
 +
<li>There were blue and white colonies but no red or green fluorescence in UV.</li>
 +
 
 +
<li>Inoculation of liquid LB medium + Amp with white colonies.</li>
 +
 
 +
</li></ol>
 +
 
 +
 
 +
<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3>
<h4>Paweł</h4>
<h4>Paweł</h4>
<p>
<p>
-
Colonies of each transformants from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p>
+
Colonies from each transformant strain from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p>
<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
-
<p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p>
+
<p>We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.</p>
</html>
</html>

Latest revision as of 14:07, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. There were blue and white colonies but no red or green fluorescence in UV.
  2. Inoculation of liquid LB medium + Amp with white colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Colonies from each transformant strain from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008"