"
Template:Team:UC Berkeley/Notebook/Molly sequencing
From 2008.igem.org
(Difference between revisions)
m (→Molly's Sequencing Log) |
(→Molly's Sequencing Log) |
||
| Line 45: | Line 45: | ||
|- | |- | ||
| MEA009 || 06/18/08 || pBca1256-K112210 || 1 || G00101 || Bad reverse read, but good part was perfect with silent mutation from AGG to AGA || [[Team:UC Berkeley/Notebook/MEA/MEA009 | MEA009]] | | MEA009 || 06/18/08 || pBca1256-K112210 || 1 || G00101 || Bad reverse read, but good part was perfect with silent mutation from AGG to AGA || [[Team:UC Berkeley/Notebook/MEA/MEA009 | MEA009]] | ||
| + | |- | ||
| + | | MA211 || 06/23/08 || pBca1256-K112210 || 1 || G00101 || Bad reverse read, two templates present || [[Team:UC Berkeley/Notebook/MEA/MA211 | MA211]] | ||
|- | |- | ||
| MEA010 || 06/15/08 || pBca1256-K112211 || 1 || ca998 || Partial read (long part) with silent mutation from AGG to AGA || [[Team:UC Berkeley/Notebook/MEA/MEA010 | MEA010]] | | MEA010 || 06/15/08 || pBca1256-K112211 || 1 || ca998 || Partial read (long part) with silent mutation from AGG to AGA || [[Team:UC Berkeley/Notebook/MEA/MEA010 | MEA010]] | ||
Revision as of 18:49, 23 June 2008
Molly's Sequencing Log
Molly's Sequencing Log
Molly's Notebook
| Read | Date | Plasmid | Clone # | Oligo | Result | File |
| MEA001 | 06/15/08 | pBca1256-K112200 | 1 | ca998 | Bad read | MEA001 |
| MA001 | 06/18/08 | pBca1256-K112200 | 2 | ca998 | Point mutation changing Asn to Lys | MA001 |
| MEA002 | 06/15/08 | pBca1256-K112201 | 1 | ca998 | Point mutation changing Asn to Lys | MEA002 |
| MA002 | 06/18/08 | pBca1256-K112201 | 2 | ca998 | Point mutation changing Asn to Lys | MA002 |
| MEA003 | 06/15/08 | pBca1256-K112202 | 1 | ca998 | Perfect | MEA003 |
| MEA004 | 06/15/08 | pBca1256-K112203 | 1 | ca998 | Perfect | MEA004 |
| MEA005 | 06/15/08 | pBca1256-K112204 | 1 | ca998 | Point mutation changing Asn to Lys | MEA005 |
| MA003 | 06/18/08 | pBca1256-K112204 | 2 | ca998 | Bad Read | MA003 |
| MEA006 | 06/15/08 | pBca1256-K112207 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA006 |
| MEA006 | 06/18/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA006 |
| AL305 | 06/23/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, 2 templates | AL305 |
| MEA007 | 06/15/08 | pBca1256-K112208 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA007 |
| MEA007 | 06/18/08 | pBca1256-K112208 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA007 |
| MA212 | 06/23/08 | pBca1256-K112208 | 1 | G00101 | Perfect read, can see silent mutation from AGG to AGA seen in forward read | MA212 |
| MEA008 | 06/15/08 | pBca1256-K112209 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA008 |
| MEA008 | 06/18/08 | pBca1256-K112209 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA008 |
| MA209 | 06/23/08 | pBca1256-K112209 | 1 | G00101 | Perfect reverse read, able to see the silent mutation from AGG to AGA seen in forward read | MA209 |
| MEA009 | 06/15/08 | pBca1256-K112210 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA009 |
| MEA009 | 06/18/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA009 |
| MA211 | 06/23/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, two templates present | MA211 |
| MEA010 | 06/15/08 | pBca1256-K112211 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA010 |
| MEA010 | 06/18/08 | pBca1256-K112211 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA010 |
| MA210 | 06/23/08 | pBca1256-K112211 | 1 | G00101 | Perfect reverse read, can see silent mutation from AGG to AGA seen in forward read | MA211 |
| MEA011 | 06/15/08 | pBca1256-K112212 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA011 |
| MEA011 | 06/18/08 | pBca1256-K112212 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA011 |
| MA208 | 06/23/08 | pBca1256-K112212 | 1 | G00101 | Not-so-great read; lots of point mutations | MA208 |
| MEA012 | 06/15/08 | pBca1256-K112213 | 1 | ca998 | Perfect | MEA012 |
| MEA013 | 06/15/08 | pBca1256-K112214 | 1 | ca998 | Perfect | MEA013 |
| MEA014 | 06/15/08 | pBca1256-K112215 | 1 | ca998 | Perfect | MEA014 |
| MEA015 | 06/15/08 | pBca1256-K112216 | 1 | ca998 | Bad read in the beginning, re-sequence & pick new colony | MEA015 |
| MEA015 | 06/18/08 | pBca1256-K112216 | 1 | ca998 | This one was perfect except it shows a deletion of A in EcoRI site, but that's probably just a problem with the sequencing data | MEA015 |
| MA005 | 06/18/08 | pBca1256-K112216 | 2 | ca998 | Perfect | MA005 |
| MEA016 | 06/15/08 | pBca1256-K112217 | 1 | ca998 | This looks like the sequencing file was switched with that for MEA017 | MEA016 |
| MEA016 | 06/18/08 | pBca1256-K112217 | 1 | ca998 | This looks like the sequencing file was switched with that for MEA017 | MEA016 |
| MEA017 | 06/15/08 | pBca1256-K112218 | 1 | ca998 | This looks like the sequencing file was switched with that for MEA016 | MEA017 |
| MEA017 | 06/18/08 | pBca1256-K112218 | 1 | ca998 | This looks like the sequencing file was switched with that for MEA016 | MEA017 |
| MEA219 | 06/20/08 | pBca1256-K1122219 | 1 | ca998 | Perfect | MEA219 |
| MEA220 | 06/20/08 | pBca1256-K1122220 | 1 | ca998 | Perfect, but there's an additional BamHI site inserted after the first one... | MEA220 |
| MEA018 | 06/15/08 | pBca1256-K112221 | 1 | ca998 | Perfect | MEA018 |
| MEA019 | 06/15/08 | pBca1256-K112222 | 1 | ca998 | Perfect | MEA019 |
| MEA223 | 06/20/08 | pBca1256-K1122223 | 1 | ca998 | Perfect | MEA223 |
| MEA224 | 06/20/08 | pBca1256-K1122224 | 1 | ca998 | Perfect | MEA224 |
| MEA020 | 06/15/08 | pBca1256-K112225 | 1 | ca998 | Perfect | MEA020 |
| MEA021 | 06/15/08 | pBca1256-K112226 | 1 | ca998 | Perfect | MEA021 |
| MEA022 | 06/15/08 | pBca1256-K112227 | 1 | ca998 | Perfect | MEA022 |
| MEA023 | 06/15/08 | pBca1256-K112228 | 1 | ca998 | This finch file aligns with K112229... | MEA023 |
| MEA024 | 06/15/08 | pBca1256-K112229 | 1 | ca998 | Bad read, re-sequence | MEA024 |
| MA004 | 06/18/08 | pBca1256-K112216 | 2 | ca998 | Insertion of an A... pick a new colony | MA004 |
| MEA025 | 06/15/08 | pBca1256-K112230 | 1 | ca998 | This part aligns with K112228... | MEA025 |
| MEA026 | Date | Plasmid | Clone # | Oligo | Result | MEA026 |
| MEA027 | Date | Plasmid | Clone # | Oligo | Result | MEA027 |
| MEA028 | Date | Plasmid | Clone # | Oligo | Result | MEA028 |
| MEA029 | Date | Plasmid | Clone # | Oligo | Result | MEA029 |
| MEA030 | Date | Plasmid | Clone # | Oligo | Result | MEA030 |
| MEA031 | Date | Plasmid | Clone # | Oligo | Result | MEA031 |
| MEA032 | Date | Plasmid | Clone # | Oligo | Result | MEA032 |
| MEA033 | Date | Plasmid | Clone # | Oligo | Result | MEA033 |
| MEA034 | Date | Plasmid | Clone # | Oligo | Result | MEA034 |
| MEA035 | Date | Plasmid | Clone # | Oligo | Result | MEA035 |
| MEA036 | Date | Plasmid | Clone # | Oligo | Result | MEA036 |
| MEA037 | Date | Plasmid | Clone # | Oligo | Result | MEA037 |
| MEA038 | Date | Plasmid | Clone # | Oligo | Result | MEA038 |
| MEA039 | Date | Plasmid | Clone # | Oligo | Result | MEA039 |
| MEA040 | Date | Plasmid | Clone # | Oligo | Result | MEA040 |
| MEA041 | Date | Plasmid | Clone # | Oligo | Result | MEA041 |
| MEA042 | Date | Plasmid | Clone # | Oligo | Result | MEA042 |
| MEA043 | Date | Plasmid | Clone # | Oligo | Result | MEA043 |
| MEA044 | Date | Plasmid | Clone # | Oligo | Result | MEA044 |
| MEA045 | Date | Plasmid | Clone # | Oligo | Result | MEA045 |
| MEA046 | Date | Plasmid | Clone # | Oligo | Result | MEA046 |
| MEA047 | Date | Plasmid | Clone # | Oligo | Result | MEA047 |
| MEA048 | Date | Plasmid | Clone # | Oligo | Result | MEA048 |
| MEA049 | Date | Plasmid | Clone # | Oligo | Result | MEA049 |
| MEA050 | Date | Plasmid | Clone # | Oligo | Result | MEA050 |
