Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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<h2 align="left">Protocols</h2>
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<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2>
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<ul>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#A_a">Purification of His_A_alpha</a></li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing various hunter/prey combinations">Testing various hunter/prey combinations</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid DNA isolation</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">DNA isolation from agarose gel</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">DNA purification after enzymatic reaction</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#genomic_DNA_isolation">Genomic DNA isolation</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">Preparation of chemocompetent bacteria</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">Preparation of electrocompetent bacteria</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Chemotransformation</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electrotransformation</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG)
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method)
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR
 
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</a></li>
 
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL)
 
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</a></li>
 
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</ul>
 
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<h4>If not stated otherwise, all agarose gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/generulers.htm#Mix">Fermentas GeneRuler (100-10000 bp)</a> ladder. PAGE gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/marksm0671.htm">PageRuler Fermentas Prestained protein ladder</a>.</h4>
 
<h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h2>
<h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h2>
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h2>
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h2>

Revision as of 16:08, 28 October 2008

Gallery Bricks Notebook Team Project Home

May
MTWTFSS
      [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_May_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_May_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_May_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_May_2008 4]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_May_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_May_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_May_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_May_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_May_2008 11]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_May_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_May_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_May_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_May_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_May_2008 18]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_May_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_May_2008 25]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_May_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_May_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_May_2008 31]
June
MTWTFSS
            [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_June_2008 1]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_June_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_June_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_June_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_June_2008 8]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_June_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_June_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_June_2008 15]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_June_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_June_2008 22]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_June_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_June_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_June_2008 29]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_July_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_July_2008 6]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_July_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_July_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_July_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_July_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_July_2008 13]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_July_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_July_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008 20]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_July_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_July_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_July_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_July_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_July_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_July_2008 27]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_July_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_July_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_July_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_August_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_August_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_August_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_August_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_August_2008 10]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_August_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_August_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_August_2008 17]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_August_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_August_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_August_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_August_2008 24]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_August_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_September_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_September_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_September_2008 7]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_September_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_September_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_September_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_September_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_September_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_September_2008 14]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_September_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_September_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_September_2008 21]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_September_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_October_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_October_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_October_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_October_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_October_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_October_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_October_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_October_2008 12]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_October_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_October_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_October_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_October_2008 19]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_October_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_October_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_October_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_October_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_October_2008 26]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_October_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_October_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_October_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_October_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_October_2008 31]
November
MTWTFSS
          [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_November_2008 7] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/16_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_November_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_November_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_November_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_November_2008 28] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 30]

Protocols

Primers

Vectors

Strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10

GM2163

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2