From 2008.igem.org
(Difference between revisions)
|
|
| Line 3: |
Line 3: |
| | ==Wednesday 3rd September== | | ==Wednesday 3rd September== |
| | | | |
| - | [[Transformations]] | + | [[Transforming into DH5α or TOP10 E. coli|Transformations]] |
| | | | |
| | * Today our aim was to transform our ligated plasmids into DH5-aplha E.coli competent cells. | | * Today our aim was to transform our ligated plasmids into DH5-aplha E.coli competent cells. |
| Line 23: |
Line 23: |
| | '''T6)''' Negative control: DH5-alpha cells only | | '''T6)''' Negative control: DH5-alpha cells only |
| | | | |
| - | * The transformations were then plated onto antibiotic [[agar]]: | + | * The transformations were then plated onto antibiotic [[Making Agar Plates|agar]]: |
| | | | |
| | '''T1 :''' +spec | | '''T1 :''' +spec |
Latest revision as of 16:35, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
Home >> Wet Lab >> Newcastle Wet Lab Journal
Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/1_August_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/2_August_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/3_August_2008&action=edit 3]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/4_August_2008 4]
| [http://2008.igem.org/Newcastle_University_Wetlab/5_August_2008 5]
| [http://2008.igem.org/Newcastle_University_Wetlab/6_August_2008 6]
| [http://2008.igem.org/Newcastle_University_Wetlab/7_August_2008 7]
| [http://2008.igem.org/Newcastle_University_Wetlab/8_August_2008 8]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/9_August_2008&action=edit 9]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/10_August_2008&action=edit 10]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/11_August_2008 11]
| [http://2008.igem.org/Newcastle_University_Wetlab/12_August_2008 12]
| [http://2008.igem.org/Newcastle_University_Wetlab/13_August_2008 13]
| [http://2008.igem.org/Newcastle_University_Wetlab/14_August_2008 14]
| [http://2008.igem.org/Newcastle_University_Wetlab/15_August_2008 15]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/16_August_2008&action=edit 16]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/17_August_2008&action=edit 17]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/18_August_2008 18]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/19_August_2008&action=edit 19]
| [http://2008.igem.org/Newcastle_University_Wetlab/20_August_2008 20]
| [http://2008.igem.org/Newcastle_University_Wetlab/21_August_2008 21]
| [http://2008.igem.org/Newcastle_University_Wetlab/22_August_2008 22]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/23_August_2008&action=edit 23]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/24_August_2008&action=edit 24]
|
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/25_August_2008&action=edit 25]
| [http://2008.igem.org/Newcastle_University_Wetlab/26_August_2008 26]
| [http://2008.igem.org/Newcastle_University_Wetlab/27_August_2008 27]
| [http://2008.igem.org/Newcastle_University_Wetlab/28_August_2008 28]
| [http://2008.igem.org/Newcastle_University_Wetlab/29_August_2008 29]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/30_August_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/31_August_2008&action=edit 31]
|
|
| September
|
| M | T | W | T | F | S | S
|
| [http://2008.igem.org/Newcastle_University_Wetlab/1_September_2008 1]
| [http://2008.igem.org/Newcastle_University_Wetlab/2_September_2008 2]
| [http://2008.igem.org/Newcastle_University_Wetlab/3_September_2008 3]
| [http://2008.igem.org/Newcastle_University_Wetlab/4_September_2008 4]
| [http://2008.igem.org/Newcastle_University_Wetlab/5_September_2008 5]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/6_September_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/7_September_2008&action=edit 7]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/8_September_2008 8]
| [http://2008.igem.org/Newcastle_University_Wetlab/9_September_2008 9]
| [http://2008.igem.org/Newcastle_University_Wetlab/10_September_2008 10]
| [http://2008.igem.org/Newcastle_University_Wetlab/11_September_2008 11]
| [http://2008.igem.org/Newcastle_University_Wetlab/12_September_2008 12]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/13_September_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/14_September_2008&action=edit 14]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/15_September_2008 15]
| [http://2008.igem.org/Newcastle_University_Wetlab/16_September_2008 16]
| [http://2008.igem.org/Newcastle_University_Wetlab/17_September_2008 17]
| [http://2008.igem.org/Newcastle_University_Wetlab/18_September_2008 18]
| [http://2008.igem.org/Newcastle_University_Wetlab/19_September_2008 19]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/20_September_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/21_September_2008&action=edit 21]
|
| [http://2008.igem.org/Newcastle_University_Wetlab/22_September_2008 22]
| [http://2008.igem.org/Newcastle_University_Wetlab/23_September_2008 23]
| [http://2008.igem.org/Newcastle_University_Wetlab/24_September_2008 24]
| [http://2008.igem.org/Newcastle_University_Wetlab/25_September_2008 25]
| [http://2008.igem.org/Newcastle_University_Wetlab/26_September_2008 26]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/27_September_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/28_September_2008&action=edit 28]
|
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/29_September_2008&action=edit 29]
| [http://2008.igem.org/Newcastle_University_Wetlab/30_September_2008 30]
|
|
Wednesday 3rd September
Transformations
- Today our aim was to transform our ligated plasmids into DH5-aplha E.coli competent cells.
- For each transformation, we added 100µl of DH5-alpha E.coli competent cells to 12.5µl of the ligation mixtures from yesterday.
- So in total we carried out 6 transformations, preparing them all on ice:
T1) Transforming pGFP-rrnB containing the 2.2kb fragment from pUC57-ncl08 into DH5-alpha
T2) Transforming pGFP-rrnB minus the 2.2kb fragment from pUC57-ncl08 into DH5-alpha
T3) Transforming pJWV021 containing the 2.2kb fragment from pUC57-ncl08 into DH5-alpha
T4) Transforming pJWV021 minus the 2.2kb fragment from pUC57-ncl08 into DH5-alpha
T5) Positive control: DH5-alpha cells plus pGFP-rrnB
T6) Negative control: DH5-alpha cells only
- The transformations were then plated onto antibiotic agar:
T1 : +spec
T2 : +spec (small and large volume plated)
T3 : +amp and +kan
T4 : +amp +kan
T5 : +spec
T6 : +spec
"
