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Team:Warsaw/Calendar-Main/19 June 2008
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| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | <p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | ||
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| + | <h3> Blue/white and rifampicin test #2 </h3> | ||
| + | <h4>Piotr, Weronika</h4> | ||
| + | <ol> | ||
| + | <li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | ||
| + | There is no mutation in beta-galactosidase gene. Why are the colonies white?</li> | ||
| + | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
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| + | Template: DNA isolated from white colonies<br> | ||
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| + | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a></ol><br> | ||
| + | <br> | ||
| + | <table id="result"> | ||
| + | <tr><th colspan="3">Colony PCR program</th></tr> | ||
| + | <tr><th>Temperature</th><th>Time</th><th>No. of cycles</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>48°C</td><td>0:45</td></tr> | ||
| + | <tr><td>72°C</td><td>1:30</td></tr> | ||
| + | <tr><td>72°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
| + | <br> | ||
</html> | </html> | ||
Latest revision as of 16:39, 28 October 2008
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Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Blue/white and rifampicin test #2Piotr, Weronika
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