"
Team:Warsaw/Calendar-Main/19 June 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
PPrzanowski (Talk | contribs) |
||
| (6 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
<html> | <html> | ||
| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | <p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | ||
| - | |||
| - | <li>The analysis of lacZ sequence from | + | <h3> Blue/white and rifampicin test #2 </h3> |
| - | There is no mutation in beta-galactosidase gene. Why | + | <h4>Piotr, Weronika</h4> |
| + | <ol> | ||
| + | <li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | ||
| + | There is no mutation in beta-galactosidase gene. Why are the colonies white?</li> | ||
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
Template: DNA isolated from white colonies<br> | Template: DNA isolated from white colonies<br> | ||
| - | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a><br> | + | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a></ol><br> |
<br> | <br> | ||
<table id="result"> | <table id="result"> | ||
Latest revision as of 16:39, 28 October 2008
 |
|
|||||||||||||||||||||||||||
 |
|
|||||||||||||||||||||||||||
Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Blue/white and rifampicin test #2Piotr, Weronika
|
|
|||||||||||||||||||||||||||
