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Team:Warsaw/Calendar-Main/19 June 2008
From 2008.igem.org
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| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
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| - | <h3> Blue/white test </h3> | + | <h3> Blue/white and rifampicin test #2 </h3> |
| - | <h4>Piotr</h4> | + | <h4>Piotr, Weronika</h4> |
| - | + | <ol> | |
<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | <li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | ||
There is no mutation in beta-galactosidase gene. Why are the colonies white?</li> | There is no mutation in beta-galactosidase gene. Why are the colonies white?</li> | ||
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Template: DNA isolated from white colonies<br> | Template: DNA isolated from white colonies<br> | ||
| - | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a><br> | + | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a></ol><br> |
<br> | <br> | ||
<table id="result"> | <table id="result"> | ||
Latest revision as of 16:39, 28 October 2008
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Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Blue/white and rifampicin test #2Piotr, Weronika
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