Team:Warsaw/Calendar-Main/19 June 2008

From 2008.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 3: Line 3:
<html>
<html>
-
<h3>Preparation of constructs with OmpA protein fusions</h3>
+
<h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
Line 9: Line 9:
-
<h3> Blue/white test </h3>
+
<h3> Blue/white and rifampicin test #2 </h3>
-
<h4>Piotr</h4>
+
<h4>Piotr, Weronika</h4>
<ol>
<ol>
<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>
<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>

Latest revision as of 16:39, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of constructs: OmpA_alpha and OmpA_omega

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white and rifampicin test #2

Piotr, Weronika

  1. The analysis of lacZ sequence from pZC320:
    There is no mutation in beta-galactosidase gene. Why are the colonies white?
  2. Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR


Colony PCR program
TemperatureTimeNo. of cycles
94°C4:00
94°C0:3028 cycles
48°C0:45
72°C1:30
72°C10:00
4°Cinfinite

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008"