Team:Warsaw/Calendar-Main/24 June 2008

From 2008.igem.org

(Difference between revisions)
 
Line 22: Line 22:
<br>
<br>
-
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=400/></a>
+
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=350/></a>
<var><b>Fig. 1.</b> PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var>
<var><b>Fig. 1.</b> PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var>

Latest revision as of 16:53, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Preparation of constructs: OmpA_alpha and OmpA_omega

Michał K.

  1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers.
  2. Gel electrophoresis (Fig. 1, no proper clones found).

Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008"