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Team:Hawaii/Protocols/Transformation E coli
From 2008.igem.org
(Difference between revisions)
(New page: == Protocol == # Thawed cells on ice. # Transformed 50 μl cells w/ 1 μl plasmid # Incubated on ice 30 min. # Heat shocked 60 sec. in 42C water bath # Added 250 μl SOC # Incubated ...) |
(→Protocol) |
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| Line 7: | Line 7: | ||
# Incubated @ 37C for 1 hour with shaking (150 rpm) | # Incubated @ 37C for 1 hour with shaking (150 rpm) | ||
# Plated 20 μl onto LB+spectinomycin plates | # Plated 20 μl onto LB+spectinomycin plates | ||
| - | * Plate more if needed. | + | :* Plate more if needed. |
Revision as of 04:28, 24 June 2008
Protocol
- Thawed cells on ice.
- Transformed 50 μl cells w/ 1 μl plasmid
- Incubated on ice 30 min.
- Heat shocked 60 sec. in 42C water bath
- Added 250 μl SOC
- Incubated @ 37C for 1 hour with shaking (150 rpm)
- Plated 20 μl onto LB+spectinomycin plates
- Plate more if needed.
