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Team:NTU-Singapore/Notebook/23 June 2008
From 2008.igem.org
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*Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between. | *Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between. | ||
*Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit. | *Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit. | ||
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| + | *Chin Chong | ||
| + | **Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel | ||
| + | **Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies | ||
| + | **Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA | ||
| + | ***allowed to incubate overnight at 37 deg cel | ||
| + | **Autoclaved MgSO4, CaCl2 and M9 salts | ||
Revision as of 15:52, 24 June 2008
Monday 23 June
Morning (9am-1230pm)
- DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
- Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
- Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
- Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
- Chin Chong
- Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel
- Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies
- Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA
- allowed to incubate overnight at 37 deg cel
- Autoclaved MgSO4, CaCl2 and M9 salts
