Team:University of Sheffield /Lab Books

From 2008.igem.org

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!align="center"|[[Team:University_of_Sheffield /Project|Our project]]
!align="center"|[[Team:University_of_Sheffield /Project|Our project]]
!align="center"|[[Team:University_of_Sheffield /Modelling|Modelling]]
!align="center"|[[Team:University_of_Sheffield /Modelling|Modelling]]
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!align="center"|[[Team:University_of_Sheffield /Parts|Parts]]
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!align="center"|[[Team:University_of_Sheffield /Wet Lab|Wet Lab]]
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!align="center"|[[Team:University_of_Sheffield /Lab Books| Our Team]]
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!align="center"|[[Team:University_of_Sheffield /Lab Books| Our team]]
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!align="center"|[[Team:University_of_Sheffield /Calendar| Calendar]]
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!align="center"|[[Team:University_of_Sheffield /Timetable| Timetable]]
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!align="center"|[[Team:University_of_Sheffield /Misc| Miscellaneous]]
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==PROTOCOLS==
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=Our Team=
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===Tecan Fluorescence Measurement===
 
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Protocol used for characterization :
 
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1. Grow 5ml of overnight cultures of DH5-alpha cells containing the plasmid with GFP-LVA.
 
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2. Resuspend the overnight cultures in 50 ml of LB medium until the OD600 reaches about 0.6.
 
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3. Quickly add 0.2 mM of IPTG to the medium and plate that into 96 well plate.
 
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4.      To 96 well plate add 180 ul of LB + IPTG as a control, 180 ul of DH5-alpha cells not induced with IPTG, finally add 180 ul of DH5-alpha induced with IPTG. Carry out the aforementioned in duplicates.
 
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5. Use the high flow cytometry machine Tecan  to measure the fluorescence every 15 minutes for 8 hours (excitation wv – 485 nm, emission wv- 535 nm )(including shaking = aeration of the cultures 2 minutes prior measurement)
 
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=Our Team=
 
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__TOC__
 
[[Image:Grouppicture.jpg|center|600px|The University of Sheffield Team]]
[[Image:Grouppicture.jpg|center|600px|The University of Sheffield Team]]
From left to right:  
From left to right:  
* Oluwaseun Samuel Awotunde
* Oluwaseun Samuel Awotunde
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* Malgorzata Poczopko
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* Malgorzata Poczopko - mba07mp@sheffield.ac.uk
* Muhammad Hammad Karim
* Muhammad Hammad Karim
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* Eva Barkauskaite
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* Eva Barkauskaite - mbb07eb@sheffield.ac.uk
* Dmitry Malyshev
* Dmitry Malyshev
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* Rosie Bavage
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* Rosie Bavage - mba07rb@sheffield.ac.uk
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=Lab Books=
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<div align=center>
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<gallery align=center perrow="3">
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Image:Dmitry_shef.jpg|[[Team:University_of_Sheffield/Dimtry | Dimtry's Lab Book]]
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Image:Eva_shef.jpg|[[Team:University_of_Sheffield/Eva | Eva's Lab Book]]
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Image:Gosia_shef.jpg|[[Team:University_of_Sheffield/Gosia | Gosia's Lab Book]]
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Image:Hammad_shef.jpg|[[Team:University_of_Sheffield/Hammad | Hammad's Lab Book]]
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Image:Rosie_shef.jpg|[[Team:University_of_Sheffield/Rosie | Rosie's Lab Book]]
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Image:SAM_shef.jpg|[[Team:University_of_Sheffield/Sam | Sam's Lab Book]]
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</gallery>
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</div>
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Latest revision as of 20:29, 28 October 2008

UniShefBanner.jpg


Introduction Our project Modelling Wet Lab Our team Timetable Miscellaneous


Our Team

The University of Sheffield Team

From left to right:

  • Oluwaseun Samuel Awotunde
  • Malgorzata Poczopko - mba07mp@sheffield.ac.uk
  • Muhammad Hammad Karim
  • Eva Barkauskaite - mbb07eb@sheffield.ac.uk
  • Dmitry Malyshev
  • Rosie Bavage - mba07rb@sheffield.ac.uk
Retrieved from "http://2008.igem.org/Team:University_of_Sheffield_/Lab_Books"