Imperial College/10 August 2008

From 2008.igem.org

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Team members -  James and Krupa + more if work load is too much.
Team members -  James and Krupa + more if work load is too much.
-
======Monday======
+
=====Monday=====
*Prepare an overnight culture of DL1-blue ''E.coli'' with the pDR110 integration vector,
*Prepare an overnight culture of DL1-blue ''E.coli'' with the pDR110 integration vector,
*Prepare an 2x overnight culture of ''B.subtilis'',
*Prepare an 2x overnight culture of ''B.subtilis'',
*Prepare the reagents required for transformation,
*Prepare the reagents required for transformation,
-
======Tuesday======
+
=====Tuesday=====
*Miniprep of the XL1-blue overnight culture,
*Miniprep of the XL1-blue overnight culture,
*Make competent cells for both of the transformation protocols
*Make competent cells for both of the transformation protocols
-
======Wednesday======
+
=====Wednesday=====
*Perform transformation of the competent cells using both protocols,
*Perform transformation of the competent cells using both protocols,
-
======Thursday======
+
=====Thursday=====
*Check successfulness of the transformation. If successful then perform transformation of linear DNA using previously made competent cells.
*Check successfulness of the transformation. If successful then perform transformation of linear DNA using previously made competent cells.
-
======Friday======
+
=====Friday=====
*Check successfulness of the linear DNA transformation,
*Check successfulness of the linear DNA transformation,
-
Produce cell number against OD<sub>600nm</sub> calibration curve for use in characterisation<font color=red> Do we want to do this next week or the following week? </font> <font color=blue>A possible activity for Friday if Wednesday's transformation is found to have failed?</font>
+
Produce cell number against OD<sub>600nm</sub> calibration curve for use in characterisation
===Cloning===
===Cloning===
Line 33: Line 33:
Chris and Tom for this week
Chris and Tom for this week
-
======Monday======
+
=====Monday=====
*Design all primers for PCR cloning
*Design all primers for PCR cloning
-
======Tuesday======
+
=====Tuesday=====
*Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015, BBa_C0012 and pSB1A3
*Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015, BBa_C0012 and pSB1A3
-
======Wednesday to Friday======
+
=====Wednesday to Friday=====
*PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome when primers arrive
*PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome when primers arrive
Line 62: Line 62:
===Thursday===
===Thursday===
*Attend microscope training (Clinton and Prudence)
*Attend microscope training (Clinton and Prudence)
 +
<br>
 +
{{Imperial/EndPage|Notebook|Notebook}}

Latest revision as of 20:34, 28 October 2008

July
MTWTFSS
  [http://2008.igem.org/wiki/index.php?title=Imperial_College/1_July_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Imperial_College/2_July_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_July_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Imperial_College/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Imperial_College/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_July_2008&action=edit 6]
[http://2008.igem.org/Imperial_College/7_July_2008 7] [http://2008.igem.org/Imperial_College/8_July_2008 8] [http://2008.igem.org/Imperial_College/9_July_2008 9] [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_July_2008&action=edit 10] [http://2008.igem.org/Imperial_College/11_July_2008 11] [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/14_July_2008&action=edit 14] [http://2008.igem.org/Imperial_College/15_July_2008 15] [http://2008.igem.org/Imperial_College/16_July_2008 16] [http://2008.igem.org/wiki/index.php?title=Imperial_College/17_July_2008&action=edit 17] [http://2008.igem.org/Imperial_College/18_July_2008 18] [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_July_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_July_2008&action=edit 20]
[http://2008.igem.org/Imperial_College/21_July_2008 21] [http://2008.igem.org/Imperial_College/22_July_2008 22] [http://2008.igem.org/Imperial_College/23_July_2008 23] [http://2008.igem.org/Imperial_College/24_July_2008 24] [http://2008.igem.org/Imperial_College/25_July_2008 25] [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_July_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_July_2008&action=edit 27]
[http://2008.igem.org/Imperial_College/28_July_2008 28] [http://2008.igem.org/Imperial_College/29_July_2008 29] [http://2008.igem.org/Imperial_College/30_July_2008 30] [http://2008.igem.org/wiki/index.php?title=Imperial_College/31_July_2008&action=edit 31]
August
MTWTFSS
        [http://2008.igem.org/Imperial_College/1_August_2008 1] [http://2008.igem.org/Imperial_College/2_August_2008 2] [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_August_2008&action=edit 3]
[http://2008.igem.org/Imperial_College/4_August_2008 4] [http://2008.igem.org/Imperial_College/5_August_2008 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_August_2008&action=edit 6] [http://2008.igem.org/Imperial_College/7_August_2008 7] [http://2008.igem.org/Imperial_College/8_August_2008 8] [http://2008.igem.org/wiki/index.php?title=Imperial_College/9_August_2008&action=edit 9] [http://2008.igem.org/Imperial_College/10_August_2008 10]
[http://2008.igem.org/Imperial_College/11_August_2008 11] [http://2008.igem.org/Imperial_College/12_August_2008 12] [http://2008.igem.org/Imperial_College/13_August_2008 13] [http://2008.igem.org/Imperial_College/14_August_2008 14] [http://2008.igem.org/Imperial_College/15_August_2008 15] [http://2008.igem.org/Imperial_College/16_August_2008 16] [http://2008.igem.org/Imperial_College/17_August_2008 17]
[http://2008.igem.org/Imperial_College/18_August_2008 18] [http://2008.igem.org/Imperial_College/19_August_2008 19] [http://2008.igem.org/Imperial_College/20_August_2008 20] [http://2008.igem.org/Imperial_College/21_August_2008 21] [http://2008.igem.org/Imperial_College/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Imperial_College/23_August_2008&action=edit 23] [http://2008.igem.org/Imperial_College/24_August_2008 24]
[http://2008.igem.org/Imperial_College/25_August_2008 25] [http://2008.igem.org/Imperial_College/26_August_2008 26] [http://2008.igem.org/Imperial_College/27_August_2008 27] [http://2008.igem.org/Imperial_College/28_August_2008 28] [http://2008.igem.org/Imperial_College/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Imperial_College/30_August_2008&action=edit 30] [http://2008.igem.org/Imperial_College/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Imperial_College/1_September_2008 1] [http://2008.igem.org/Imperial_College/2_September_2008 2] [http://2008.igem.org/Imperial_College/3_September_2008 3] [http://2008.igem.org/Imperial_College/4_September_2008 4] [http://2008.igem.org/Imperial_College/5_September_2008 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_September_2008&action=edit 6] [http://2008.igem.org/Imperial_College/7_September_2008 7]
[http://2008.igem.org/Imperial_College/8_September_2008 8] [http://2008.igem.org/Imperial_College/9_September_2008 9] [http://2008.igem.org/Imperial_College/10_September_2008 10] [http://2008.igem.org/Imperial_College/11_September_2008 11] [http://2008.igem.org/Imperial_College/12_September_2008 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Imperial_College/14_September_2008&action=edit 14]
[http://2008.igem.org/Imperial_College/15_September_2008 15] [http://2008.igem.org/Imperial_College/16_September_2008 16] [http://2008.igem.org/Imperial_College/17_September_2008 17] [http://2008.igem.org/Imperial_College/18_September_2008 18] [http://2008.igem.org/Imperial_College/19_September_2008 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Imperial_College/21_September_2008&action=edit 21]
[http://2008.igem.org/Imperial_College/22_September_2008 22] [http://2008.igem.org/Imperial_College/23_September_2008 23] [http://2008.igem.org/Imperial_College/24_September_2008 24] [http://2008.igem.org/Imperial_College/25_September_2008 25] [http://2008.igem.org/Imperial_College/26_September_2008 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_September_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Imperial_College/28_September_2008&action=edit 28]
[http://2008.igem.org/Imperial_College/29_September_2008 29] [http://2008.igem.org/Imperial_College/30_September_2008 30]

10 August 2008

Wetlab Team

B.subtilis

Team members - James and Krupa + more if work load is too much.

Monday
  • Prepare an overnight culture of DL1-blue E.coli with the pDR110 integration vector,
  • Prepare an 2x overnight culture of B.subtilis,
  • Prepare the reagents required for transformation,
Tuesday
  • Miniprep of the XL1-blue overnight culture,
  • Make competent cells for both of the transformation protocols
Wednesday
  • Perform transformation of the competent cells using both protocols,
Thursday
  • Check successfulness of the transformation. If successful then perform transformation of linear DNA using previously made competent cells.
Friday
  • Check successfulness of the linear DNA transformation,

Produce cell number against OD600nm calibration curve for use in characterisation

Cloning

Chris and Tom for this week

Monday
  • Design all primers for PCR cloning
Tuesday
  • Transform XL1-blue E.coli with Biobricks containing BBa_B0015, BBa_C0012 and pSB1A3
Wednesday to Friday
  • PCR clone AmyE from pDR111 and XylR from the B.subtilis genome when primers arrive
  • Prepare ALL remaining Protocols
  • Depending on arrival of primers, connect XylR and BBa_C0012 to BBa_B0015 (separately) to begin construction of inducible promoters

Microscope

  • Prepare B.subtilis for first microscopy session on thursday - a set of dilutions to ascertain optimal amount of cells per mL
  • Chris, James, Prudence and Clinton will attend microscope training

Drylab Team

End of week five

Finish tutorial 2:

  • Maximum Likelihood Estimation
  • Moments
  • The Bayesian Approach

Next week

  • Start working on tutorial 3 (hypothesis testing)
  • Generate data sets from bacteria motility movies

Thursday

  • Attend microscope training (Clinton and Prudence)



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