Imperial College/14 August 2008
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==Wet Lab== | ==Wet Lab== | ||
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+ | === Cloning === | ||
*Parts taken from the registry and transformed by electroporation into XL1-Blue ''E.coli'' along with XL1-Blue controls and grown on Kanamycin and Ampicillin plates. Parts taken: | *Parts taken from the registry and transformed by electroporation into XL1-Blue ''E.coli'' along with XL1-Blue controls and grown on Kanamycin and Ampicillin plates. Parts taken: | ||
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*Chris, Clinton, James and Prudence received microscope training on Widefield 1, recording two 60s videos of ''B.subtilis'' swimming for analysis | *Chris, Clinton, James and Prudence received microscope training on Widefield 1, recording two 60s videos of ''B.subtilis'' swimming for analysis | ||
- | *Determined that best results would be obtained by bringing a ''B.subtilis'' overnight culture to the microscope facility and diluting 100 fold | + | *Determined that best results would be obtained by bringing a ''B.subtilis'' overnight culture to the microscope facility and diluting 100 fold. |
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+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:36, 28 October 2008
14 August 2008Wet LabCloning
B.subtilis
Dry Lab
Microscope
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