"
Imperial College/28 August 2008
From 2008.igem.org
(Difference between revisions)
m |
|||
| (3 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
| - | | rowspan="2" bgcolor=# | + | | rowspan="2" bgcolor=#ffffff width="100%" | |
|} | |} | ||
= 28 August 2008 = | = 28 August 2008 = | ||
==WetLab== | ==WetLab== | ||
*Performed midi preps for Terminator, GFP-T, RFP-T and AK3, all had high concentrations and good yields | *Performed midi preps for Terminator, GFP-T, RFP-T and AK3, all had high concentrations and good yields | ||
| - | + | [[Image:28-8.PNG|center|frame| Lanes : M = Marker, 1 = GFP-mut3b - terminator Midi 1 Digested, 2 = GFP-mut3b - terminator Midi 1 Undigested, 3 = GFP-mut3b - terminator Midi 2 Digested, 4 = GFP-mut3b - terminator Midi 2 Undigested, 5 = mRFP - terminator Midi 1 Digested, 6 = mRFP - terminator Midi 1 Undigested, 7 = mRFP - terminator Midi 2 Digested, 8 = mRFP - terminator Midi 2 Undigested, 9 = CAT midi 1 Digested , 10 = CAT midi 1 Undigested, 11 = CAT midi 2 Digested, 12 = Catmidi 2 Undigested ]] | |
| + | *All insert and vector sizes appear to be correct with minimal contamination | ||
*Double digests with Spe and EcoRI were performed on GFP-T and RFP-T and a 1% agarose gel run. The AK3 and terminator constructs will be digested and run a gel tomorrow morning. | *Double digests with Spe and EcoRI were performed on GFP-T and RFP-T and a 1% agarose gel run. The AK3 and terminator constructs will be digested and run a gel tomorrow morning. | ||
*Optimised vector genomics and single colony PCR protocols was also performed today. The results of these will be written out in more detail tomorrows notebook. | *Optimised vector genomics and single colony PCR protocols was also performed today. The results of these will be written out in more detail tomorrows notebook. | ||
| + | |||
| + | ==DryLab== | ||
| + | *Completed production of synthetic video. Updated wiki with regards to generation of synthetic trajectory and bacteria characteristics used to create synthetic video. | ||
| + | <br> | ||
| + | {{Imperial/EndPage|Notebook|Notebook}} | ||
Latest revision as of 20:40, 28 October 2008
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
28 August 2008WetLab
 Lanes : M = Marker, 1 = GFP-mut3b - terminator Midi 1 Digested, 2 = GFP-mut3b - terminator Midi 1 Undigested, 3 = GFP-mut3b - terminator Midi 2 Digested, 4 = GFP-mut3b - terminator Midi 2 Undigested, 5 = mRFP - terminator Midi 1 Digested, 6 = mRFP - terminator Midi 1 Undigested, 7 = mRFP - terminator Midi 2 Digested, 8 = mRFP - terminator Midi 2 Undigested, 9 = CAT midi 1 Digested , 10 = CAT midi 1 Undigested, 11 = CAT midi 2 Digested, 12 = Catmidi 2 Undigested
DryLab
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
