Imperial College/31 August 2008
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|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
- | | rowspan="2" bgcolor=# | + | | rowspan="2" bgcolor=#ffffff width="100%" | |
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- | = | + | =31 August 2008= |
==Wet Lab== | ==Wet Lab== | ||
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* Whenever the sequences from GeneArt arrive, priority becomes the rapid cloning of sequences into Biobricks to begin construction | * Whenever the sequences from GeneArt arrive, priority becomes the rapid cloning of sequences into Biobricks to begin construction | ||
- | ====Monday==== | + | =====Monday===== |
*Test all primers for PCR cloning from pDR111 using taq to ascertain optimal consitions. | *Test all primers for PCR cloning from pDR111 using taq to ascertain optimal consitions. | ||
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*If time allows, test genomic primers using ''B.subtilis'' chromosome sample 1 or using single colony PCR | *If time allows, test genomic primers using ''B.subtilis'' chromosome sample 1 or using single colony PCR | ||
- | ====Tuesday==== | + | =====Tuesday===== |
*Carry out PCR cloning with Pfu, purify and clone DNA sequences into Biobricks | *Carry out PCR cloning with Pfu, purify and clone DNA sequences into Biobricks | ||
- | ====Wednesday==== | + | =====Wednesday===== |
- | Test orientation of inserts to find a correct Biobrick containing host to culture overnight for midiprepping | + | *Test orientation of inserts to find a correct Biobrick containing host to culture overnight for midiprepping |
- | ====Thursday | + | =====Thursday & Friday===== |
*Hopefully, construction begins... | *Hopefully, construction begins... | ||
- | ===''B.subtilis''=== | + | =====''B.subtilis''===== |
- | ====Monday==== | + | =====Monday===== |
*Prepare all media and plates needed for the rest of the week, | *Prepare all media and plates needed for the rest of the week, | ||
*Carry out single colony PCR on ''B.subtilis'' transformed previously with pDR111 using verification primers. These verification primers work on the basis of flanking the chromosomal integration sites allowing the integration sites and any inserted elements to be amplified. | *Carry out single colony PCR on ''B.subtilis'' transformed previously with pDR111 using verification primers. These verification primers work on the basis of flanking the chromosomal integration sites allowing the integration sites and any inserted elements to be amplified. | ||
- | ====Tuesday==== | + | =====Tuesday===== |
*Prepare overnight culture for the ''B.subtilis'' growth curve | *Prepare overnight culture for the ''B.subtilis'' growth curve | ||
*Perform a transformation using linear DNA derived from pDR110 or pDR111 | *Perform a transformation using linear DNA derived from pDR110 or pDR111 | ||
- | ====Wednesday==== | + | =====Wednesday===== |
*2nd trial of the ''B.subtilis'' growth curve and OD<sub>600</sub> vs c.f.u. experiment | *2nd trial of the ''B.subtilis'' growth curve and OD<sub>600</sub> vs c.f.u. experiment | ||
*Perform single colony PCR with the ''B.subtilis'' transformed yesterday using verifications primers. | *Perform single colony PCR with the ''B.subtilis'' transformed yesterday using verifications primers. | ||
*Begin to try to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA. This includes the volume of transformed cells, amount of DNA, the density of competent cells and length of DNA incubation. | *Begin to try to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA. This includes the volume of transformed cells, amount of DNA, the density of competent cells and length of DNA incubation. | ||
- | ====Thursday==== | + | =====Thursday===== |
<br> | <br> | ||
- | ====Friday==== | + | =====Friday===== |
*Carry on to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA | *Carry on to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA | ||
+ | |||
+ | ==Dry Lab== | ||
+ | |||
+ | =====ODE team Weekly plan===== | ||
+ | |||
+ | *Develop the MATLAB simulation of the genetic circuitry: simulate behaviour of the constituive and inducible gene expression of the genetic parts. | ||
+ | *Develop the characterisation of the ''B.Subtilis'' chassis; simulate an ODE system regarding the growth of the ''B.Subtilis'' (model the process of the diffusion of nutrients into the bacteria, additionally simulating the lag phase and the stationary phase of the growth) | ||
+ | *Present a list of parameters for wet lab | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:42, 28 October 2008
31 August 2008Wet LabCloning
Monday
Tuesday
Wednesday
Thursday & Friday
B.subtilisMonday
Tuesday
Wednesday
Thursday
Friday
Dry LabODE team Weekly plan
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